FastQCFastQC Report
Thu 5 Oct 2023
SRR7471246.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471246.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189705939
Total Bases9.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA120866936.3712781285144695Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA79398194.18532969597752No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT33079581.7437292777639397No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA30278291.5960644226325462Illumina Single End Adapter 1 (95% over 22bp)
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA17543940.9247965610607478No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG17137200.9033560093234614No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG16262480.8572467517740706No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG14220280.7495959312059282No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA13948390.7352637494390727Illumina Single End Adapter 1 (95% over 22bp)
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA13256070.6987693727395641No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATC10779540.5682236442792653No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG9096990.4795311126237329No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT8341900.43972793071069854No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG8303550.4377063809267458No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA7779800.410097862038995No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT7716720.40677271574507745No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC7630010.4022019574200047No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG7610720.4011851205143345No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA7167680.3778310809763315No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC6835710.36033189240322094No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC6633600.34967803511939605Illumina Single End Adapter 1 (95% over 22bp)
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATC6292260.3316849242131529No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA6064250.3196657960191747No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCAT5744490.3028102351608507No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT5641600.29738657786565137No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTATGCCGT5389240.2840838841634789No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT5285940.2786386144716323Illumina Single End Adapter 1 (95% over 22bp)
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTC5233850.2758927858341852No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT5229740.27567613473608754No Hit
TTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCAC5177030.27289762393785677No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC5106960.2692040126376855No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC5096880.2686726639591394No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT4888030.2576635199596993No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT4825610.2543731643530675No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG4366100.23015093902779712No Hit
AGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGTCTTCTG4048670.21341819983822433No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4018280.21181624682820288Illumina Single End Adapter 1 (95% over 22bp)
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT3942010.20779581392019572No Hit
TATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGT3904150.20580009358589454No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCAT3879970.20452548931533449No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG3873780.20419919483912413Illumina Single End Adapter 1 (95% over 22bp)
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCATC3864550.2037126523487491No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA3794290.2000090255476925No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT3655040.1926687176620232No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTATGC3644210.19209783411156148No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT3369260.1776043500672902No Hit
TTCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGC3364060.17733024162200847No Hit
GACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3354980.17685160610601652Illumina Single End Adapter 1 (95% over 22bp)
TTGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA3283020.17305836692861787No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT3217770.16961883307195774No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATT3180640.16766159334632114No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT3156150.1663706479953693No Hit
TCTACATGGTATAACTGTGGTAATTCTCTGTAGGCACCATCAATTCGTAT3114390.16416934632710684No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC3079080.1623080445573188No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG2864090.150975241739796No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT2806010.14791366125864938No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT2673550.14093127574672293No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA2665440.14050377199840855No Hit
TTTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTA2656060.14000932253365037No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCA2614360.13781118365514114No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAA2555270.13469636287981476No Hit
TTGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTAT2332450.12295081599949277No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT2329380.12278898659045145No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA2293710.1209087080821439No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAAT2247910.11849444523716254No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA1998180.10533038715250766Illumina Single End Adapter 1 (95% over 22bp)
AGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTATGCCG1987550.10477004623455674No Hit
TGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGT1970790.10388657363014872No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCA1960240.10333044976520213No Hit
TTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTAT1930640.10177014015359846No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATTCGTATGC1927700.10161516345568918No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG1924100.10142539607049413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)