FastQCFastQC Report
Sun 1 Oct 2023
SRR7471247.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471247.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19863403
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT10652565.362907856221817No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA4709832.3711093209960046No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC4590862.311215253499111No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT3838401.9323979884010811No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC2262301.138928712265466No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG2137151.075923395402087No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG2135261.074971896809424No Hit
CCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCCG2134921.0748007277504263No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC1665710.8385823919496573No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG1660620.8360198904487816No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG1587870.7993947462073846No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC1316430.6627414245182459No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT1277440.6431123609584923No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA1262230.6354550627603941No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT1228430.6184388445423978No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT1216670.6125184088547164No Hit
AGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGTCTTCTG1191800.5999978956274511No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT1107760.5576889317505163No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1104520.556057791305951No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT1036060.5215923978383764No Hit
TTCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGC1000680.5037807469344503No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG996470.5016612712333329No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT911260.4587632844180828No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA857520.4317085043282865No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA737410.3712405170453421Illumina Single End Adapter 1 (95% over 22bp)
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT733800.3694231043895147No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC712000.358448147077316No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG614580.30940317729041694No Hit
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGCC543120.27342746859639305No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG527890.2657601016301185No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA524900.26425482078775725No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT521500.2625431301977813No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC517950.26075592384648294No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT447550.22531385986580446No Hit
TCCAACGGGGCCTTTCCTTCTGGCTGTAGGCACCATCAATTCGTATGCCG385470.19406040344647893No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT379370.19098942915269856No Hit
TTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATTCGTATGCC377060.18982648642833255No Hit
TCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT355620.17903276694330775No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT351910.17716501044659871No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATTCGTATGCC329340.16580240555961132No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATTC328070.1651630387804144No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG319450.16082339969641657No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG310710.15642334800336077No Hit
GTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT307470.15479220755879544No Hit
AGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGC302430.1522548779783605No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTC293210.14761317584907277No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATTCGTATGC290660.14632940790659082No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC289820.145906519643185No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATTCGTATGC277310.13960850514889114No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT276770.13933664840813026No Hit
TTGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTAT276530.13921582319001433No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC273390.13763502658633064No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAAT271620.13674394060272552No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATG263010.13240933590281584No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT260690.13124135879436166No Hit
TGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTATGC250870.12629759361978407No Hit
TTGCCTTGTTGTAGACGGCCTTGGTCTGTAGGCACCATCAATTCGTATGC248590.12514975404768258No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT243840.1227584216058044No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT242340.12200326399257973No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT240370.12101149032721131No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT240330.12099135279085864No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTC231290.11644026957515789No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAATT226510.11403383398101523No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATTCG225840.1136965302471082No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT220600.11105851298490999No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATT208730.10508269907225866No Hit
TGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGT207920.10467491396111733No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATTCGTATGC205970.10369320906392526No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG199640.10050644393611709No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)