FastQCFastQC Report
Sun 1 Oct 2023
SRR7471248.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471248.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26600083
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA301365511.329494723757065Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8793123.305673895829573Illumina Single End Adapter 1 (95% over 22bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT8278723.112291040595625No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3500281.3158906308675804Illumina Single End Adapter 1 (95% over 22bp)
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG2850641.0716658290126388No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG2562400.9633052648745495No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG2078420.7813584641822358No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA1936140.7278699092781027No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC1545490.5810094652712174Illumina Single End Adapter 1 (95% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1519410.5712049845859504No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1372400.5159382397415827No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1324020.49775032656852986Illumina Single End Adapter 1 (95% over 22bp)
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA1286800.4837578890261357No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG1261580.47427671560272955No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG1176530.44230313115940273No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA1121260.4215250005046977No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA1103010.4146641196570703No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC1011970.3804386625410154No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG969780.3645778097760071No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC954790.3589424890140381No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA931200.350074095633461Illumina Single End Adapter 1 (95% over 22bp)
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT930510.3498146979466192No Hit
GACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA909470.34190494819132705Illumina Single End Adapter 1 (95% over 22bp)
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC893250.33580722285716175No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA887610.33368692872123745No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG877530.3298974668612876Illumina Single End Adapter 1 (95% over 22bp)
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC859000.32293132318421713No Hit
AGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGTCTTCTG831680.312660678539988No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT815950.30674716315734807No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA807220.30346521851078434No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT780100.29326976160187174No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT773590.29082240081732075No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT755320.28395400119616165No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT754150.28351415294456034No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT708650.2664089431600646No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT673060.2530292856604996No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT646530.24305563257077056No Hit
CCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCCG593470.2231083263913124No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT573580.21563090611408994No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT527490.1983038925104106No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTC499780.18788663178231435No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAAT487120.18312724813678213No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA477930.17967237169899056Illumina Single End Adapter 1 (95% over 22bp)
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG435520.16372881242513415No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA431270.1621310730496593No Hit
TCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA430470.1618303221083934Illumina Single End Adapter 1 (95% over 22bp)
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC416320.15651078983475353No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT409270.15386042216484813No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA396140.1489243473413222Illumina Single End Adapter 1 (95% over 22bp)
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATC393490.14792810984837904No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT382750.14389052846188488No Hit
TGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGT378810.1424093300761505No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATTC357290.1343191297560989No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG338320.1271875730613322No Hit
TTCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGC335300.12605223825805356No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA299170.11246957387313415Illumina Single End Adapter 1 (95% over 22bp)
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC290160.10908236639712741No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG288950.10862748059846279No Hit
ATGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTC288560.10848086451459568No Hit
TTGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTAT287360.10802973810269689No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA283970.10675530598908281No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCA283900.10672899028172206No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATT283390.10653726155666506No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT277640.10437561416631669No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT276410.10391320959412043No Hit
GTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT273310.10274779969671523No Hit
TATGCGAAAGCAGTTGAAGACAAGTTCTGTAGGCACCATCAATTCGTATG272930.10260494299961394No Hit
TAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGTCTTCT267140.10042825806220229No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)