FastQCFastQC Report
Sun 1 Oct 2023
SRR7471249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7705913
Total Bases220.5 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA519750.67448205034238No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA516240.669927106625782No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG412060.5347322244619164No Hit
AAGGCCGAGAGCTGATGACGAGTTAAC404990.5255574517905924No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA357360.4637477739496929No Hit
AAGAGGTGCACAATCGACCGATCCTGA342260.444152432034984No Hit
AGAGATGTATTTATTAGATAAAAAATCA328050.4257120473589566No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC310680.4031709156332287No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCA295000.382822904956233No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT294060.38160306247942327No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC289260.3753740796191185No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT274910.35675201627633224No Hit
AAGGCCGAGAGCTGATGACGAGTTACCT239610.31094303815784063No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC236040.3063102321554889No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG230820.2995362132949074No Hit
ACGCGAAACTCAGGTGCTGCAATCT229320.2975896561510622No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG211110.2739584524247808No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCT187420.24321582659965146No Hit
TTGACGTGGGTTAGTCGATCCTAAGAGATGGG177050.22975862821186793No Hit
TACGCGAAACTCAGGTGCTGCAATCT167950.2179495148725401No Hit
TAGAGATGTATTTATTAGATAAAAAATCA162390.210734276392687No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT156340.20288316257917782No Hit
AGAGATGTATTTATTAGATAAAAAATC155260.20148164143560923No Hit
TCCAACGGGGCCTTTCCTTCTGGC154840.2009366054353326No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCC143390.18607788590398047No Hit
TCCAACGGGGCCTTTCCTTCTGGCT141530.18366415504561237No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG140160.18188629952090038No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC133210.17286725142108406No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCT131330.17042756646746465No Hit
TTGGAAGAGATGTATTTATTAGATAAA129480.16802681265672217No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC119950.15565968626949203No Hit
AAGGCCGAGAGCTGATGACGAGTTACC119860.15554289284086129No Hit
TCGTGGGTTAGTCGATCCTAAGAGATGGG118160.15333679474450335No Hit
AAAAAAAAAAAAAAA112840.14643300540766552No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCA109290.14182615350056507No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCC109260.14178722235768818No Hit
TTGACGTGGGTTAGTCGATCCTAAGAGATGGGG105760.13724525568871593No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT104260.13529869854487067No Hit
TTGGAAGAGATGTATTTATTAGATA103680.13454602978258384No Hit
TTTCCAACGGGGCCTTTCCTTCTGGC100940.13099031873315983No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA100590.13053612206626264No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGG99450.12905673863694023No Hit
TAGAGATGTATTTATTAGATAAAAAATC96250.12490408339673703No Hit
AGATGTATTTATTAGATAAAAAATCAATG96230.12487812930148576No Hit
TTTGGAAGAGATGTATTTATTAGATAAA95380.12377508025330677No Hit
TACCATACGCGAAACTCAGGTGCTGCAATCT95240.12359340158654789No Hit
TATACGCGAAACTCAGGTGCTGCAATCT94940.12320409015777883No Hit
TGGACGTGGGTTAGTCGATCCTAAGAGATGGG91620.11889571034606802No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT91520.11876593986981165No Hit
TGATGTATTTATTAGATAAAAAATCAATG90940.11801327110752484No Hit
TAGCGTGTTACCTATACTCTACCGTCAGGGT89760.1164819794876999No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC89730.116443048344823No Hit
TTGGAAGAGATGTATTTATTAGATAAAA87140.11308199300978353No Hit
TTACGCGAAACTCAGGTGCTGCAATCT84510.10966902948424152No Hit
TTGGAAGAGATGTATTTATTAGATAA83590.1084751411026831No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG82470.10702171176861199No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC81890.10626904300632516No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCA81650.10595759386330991No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG80430.10437439405298243No Hit
TTGCCTTGTTGTAGACGGCCTTGGTAGGTC78960.10246676805201409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)