FastQCFastQC Report
Sun 1 Oct 2023
SRR7471251.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471251.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42943161
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1166642527.167131455460392No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATTCGTATGC13115363.0541207714075824No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG13045693.0378970006423143No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT10833132.522667113396706No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA10710172.494033916133933Illumina Single End Adapter 1 (95% over 22bp)
TCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT7444701.7336171410390586No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG6648291.5481603694707058No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA5540191.29012160981815No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA4823641.123261513049773No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA4806081.119172386960522No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA4254130.9906420256301113No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT3835130.8930711924070984No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCC3822460.8901207808153667No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA3099320.7217260974337683No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT2575900.5998394016686383No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1939760.4517040559729639Illumina Single End Adapter 1 (95% over 22bp)
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA1744110.40614383277467625No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC1670410.38898161222924416No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT1646890.38350460507553225No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC1637600.38134128039619625No Hit
ATGATTAATAGGGACGGTCGGGGGCTGTAGGCACCATCAATTCGTATGCC1529980.35628024681275794No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT1396090.3251018247119722No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC1386260.3228127524194132No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTA1259820.2933691816492037No Hit
AAGAGGTGCACAATCGACCGATCCTGATCTGTAGGCACCATCAATTCGTA1247630.2905305457136702No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT1224290.2850954544310327No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATTCGTATGCCG1168860.2721876947996446No Hit
CTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTATG1042960.24286987164265808No Hit
TTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATTCGTATGCC1002490.23344578662944723No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT951090.2214764767782232No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATTCGTATG861440.20060004432370498No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATGC853600.1987743752724677No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG843190.19635024072866925No Hit
ATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTATGCC843000.19630599619809078No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATTCGTAT806110.1877155712873582No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATG764700.17807259228075922No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT751870.17508492213696145No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA708370.1649552532939995No Hit
TATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATTCGTATG689830.16063791857334397No Hit
CCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT672590.15662330958822523No Hit
TTGCCTTGTTGTAGACGGCCTTGGTCTGTAGGCACCATCAATTCGTATGC662060.1541712311303772No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT644870.15016826544277911No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT636310.14817493290724454No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA635070.1478861791287325Illumina Single End Adapter 1 (95% over 22bp)
CCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCCG610960.14227178106427704No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCG602080.14020393142461032No Hit
GCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT570790.13291755583619008No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG544350.12676058010727248No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATTCG534310.12442260596512679No Hit
AAGAGGTGCACAATCGACCGATCCTGCCTGTAGGCACCATCAATTCGTAT530660.12357264524611962No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACATCAATTCGTATG527430.1228204882262859No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATTCGTATGC520720.12125795769901522No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC507820.11825398693868856No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT500170.11647256241802972No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG499530.11632352820976546No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT493210.11485181540315582No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCCTGTAGGCACCATCAATTCGT445670.10378136812052563No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA442840.10312235748085707No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC434670.10121984266598352No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG433000.10083095652879395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)