FastQCFastQC Report
Sun 1 Oct 2023
SRR7471252.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471252.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54702770
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT868688515.880155611863897No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA26699704.88086800723254Illumina Single End Adapter 1 (95% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG21670903.9615726954960415No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG11078092.0251424196617465No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATTCGTATGC7149761.3070197359292774No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6872841.2563970709344336Illumina Single End Adapter 1 (95% over 22bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCC5674031.0372472911335202No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATT4316550.7890916675700336No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA4012040.7334253822978252No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG3224120.5893888006036988No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA2806660.5130745664250641No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT2758980.5043583716144538No Hit
TCGATGAAACCCTGGTTCGATTCTAGGAGATGGCCTGTAGGCACCATCAA2719140.49707537662169576No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2462440.45014905095299557Illumina Single End Adapter 1 (95% over 22bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATGC2327860.425547006120531No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT1876310.343000912019629No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC1871180.3420631167306518No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAAT1679410.30700639108403466No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1597040.29194865269162784No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATGC1573690.28768013027493855No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCG1523540.27851240439926533No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT1474770.26959695094050995No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTCG1373600.2511024578828458No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG1349570.2467096273186897No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTA1314200.24024377558942628No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATGCCGT1311490.2397483710605514No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCTGTAGGCACCATCAA1287250.23531715121556002No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1199040.21919182520373282No Hit
TCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT1089770.19921660274242053No Hit
ATGATTAATAGGGACGGTCGGGGGCTGTAGGCACCATCAATTCGTATGCC1083550.19807954880529818No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAATCTGTAGGCACCATCAA1070810.19575059910128864No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC1067810.1952021808036412No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACTGTAGGCACCATCA1052030.1923175005580156No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCTGTAGGCACCATCA1049450.1918458608220388No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT1008660.18438920003502565No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTATG944350.17263293979445649No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCACTGTAGGCACCATC930950.17018333806496452No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT900780.16466807805162334No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCCTGTAGGCACCAT895410.1636864092988344No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA887000.1621490100044294No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT819270.14976755290454213No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC808340.14776948224011324No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC790020.1444204745024795No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATTCGTATGCCG781930.14294157315982353No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT760350.1389966175387462No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTC750490.13719414940047825No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT738290.1349639149900453No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT726960.13289272188593007No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATT726140.13274282088457312No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATCA709880.12977039371132393No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT688160.1257998452363564No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA687550.12568833351583475No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATGCCGTCT687080.12560241464920333No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT675840.12354767409401754No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG640210.117034292778958No Hit
GCCAAGGACGTTTTCATTAATCAAGAACCTGTAGGCACCATCAATTCGTA636660.11638533112674183No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT574320.10498919890162785No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGTCTGTAGGCACCATCAAT572710.10469488108189037No Hit
TCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCTGTAGGCACCATC558540.10210451865600223No Hit
ACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA555300.10151222689454299Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)