FastQCFastQC Report
Sun 1 Oct 2023
SRR7471253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37299383
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length20-45
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGAAACTCAGGTGCTGCAATCT25554236.851113328067653No Hit
ATGTATTTATTAGATAAAAAATCAATG24794296.647372692465181No Hit
ATGTACACGGAGTCGACCCGCAACGCGA19050925.107569741837285No Hit
ACGTTCTAGCATTCAAGGTCCCA16418814.401898551512233No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA12968813.476950275558177No Hit
AGAGATGTATTTATTAGATAAAAAATCA12016073.2215197768821002No Hit
ACGTTCTAGCATTCAAGGTCCC9848122.6402903233010586No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC9304162.494454130782807No Hit
AAGAGGTGCACAATCGACCGATCCTGA7795892.0900855116021626No Hit
AGAGATGTATTTATTAGATAAAAAATC5768681.546588585661055No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA5534451.4837913002475134No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA4918071.3185392369627134No Hit
TGATGTATTTATTAGATAAAAAATCAATG4683641.2556883313592613No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA4521301.2121648232090059No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT4402371.1802795772787984No Hit
ACTATGCCGACTAGGGATCGGGTGGTG4149551.112498295213087No Hit
TACGCGAAACTCAGGTGCTGCAATCT3841631.029944650827066No Hit
ATGTATTTATTAGATAAAAAATCA3224210.8644137625547319No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC2850520.7642271187166823No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT2732380.7325536725366207No Hit
TGATGTATTTATTAGATAAAAAATCA2317100.6212167102067078No Hit
ATGTATTTATTAGATAAAAAATCAAT2125920.5699611706713754No Hit
ATTTATTAGATAAAAAATCAATG2060850.5525158418840332No Hit
TCCAACGGGGCCTTTCCTTCTGGC2002870.5369713488290142No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC1853750.4969921352318348No Hit
ACGTTCTAGCATTCAAGGTCC1726740.462940633629248No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC1361100.3649122024350912No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT1308980.35093878094444614No Hit
TTTGGAAGAGATGTATTTATTAGATAA1166640.31277729178522873No Hit
TCCACGTTCTAGCATTCAAGGTCCCA1132530.30363236839601343No Hit
TTTGGAAGAGATGTATTTATTAGATA1111300.29794058523702655No Hit
ATTACCCAATCCTAATTCAGGGAGGTAG1037540.27816545919807845No Hit
TAGAGATGTATTTATTAGATAAAAAATCA981660.26318397813711825No Hit
TTGGAAGAGATGTATTTATTAGATAA915320.2453981611438452No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG908380.243537540553955No Hit
ATGTATTTATTAGATAAAAAATCAA898460.24087797913440015No Hit
ACGGAGTCGACCCGCAACGCGA859580.2304542142158223No Hit
ATGTATTTATTAGATAAAAAATC849380.2277195845303929No Hit
TTCCACGTTCTAGCATTCAAGGTCCCA847270.2271538915268384No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCT825560.22133341991206665No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA816090.21879450391981015No Hit
ATTTATTAGATAAAAAATCA799490.21434402815724862No Hit
AGAGATGTATTTATTAGATAAAAAATCAA788880.21149947708250294No Hit
AACGGGGCCTTTCCTTCTGGCTAACCT747750.2004724850274333No Hit
CAAGAGGTGCACAATCGACCGATCCTGA725210.19442948962453346No Hit
TGCTTGGTGGGGCTTGCTCTGCTAGGCG682900.18308613844899257No Hit
TGATGTATTTATTAGATAAAAAATCAAT664560.17816916703421073No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCT653290.1751476693327608No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGA646520.17333262590429446No Hit
TCACGTTCTAGCATTCAAGGTCCCA640850.17181249352033517No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC638130.17108325893755402No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCA629820.1688553400467777No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA622190.1668097298016967No Hit
CCAGATCGGGGTTCAATTCCCCGTCGCGGA615960.16513946088598838No Hit
TTACGCGAAACTCAGGTGCTGCAATCT594530.15939405753709115No Hit
TTGGAAGAGATGTATTTATTAGATA585430.15695433889616886No Hit
TCGTATGGCGCAGTGGTAGCGCAGCAGA584090.1565950836237693No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC576310.15450925823625555No Hit
TCCACGTTCTAGCATTCAAGGTCCC575080.1541794940683067No Hit
ACGGGGCCTTTCCTTCTGGCTAACCT561440.1505225971164188No Hit
AGATCGGGGTTCAATTCCCCGTCGCGGA554410.14863784744106892No Hit
TAGAGATGTATTTATTAGATAAAAAATC550350.14754935758588822No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG531440.1424795686298618No Hit
ACACGGAGTCGACCCGCAACGCGA526930.1412704333473827No Hit
GTCTAGTCGGTTATGGCATCTGC514620.13797011065839881No Hit
TTTCCACGTTCTAGCATTCAAGGTCC505280.1354660477895841No Hit
AGATGTATTTATTAGATAAAAAATCA493770.1323802058602417No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT485210.13008526173207746No Hit
AAGAGGTGCACAATCGACCGATCCTGAT480300.12876888606977763No Hit
TTGGAAGAGATGTATTTATTAGATAAA472570.12669646572974141No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGC471170.12632112440036877No Hit
TTTGGAAGAGATGTATTTATTAGATAAA463920.1243773925161175No Hit
AGAGATGTATTTATTAGATAAAAAA461790.12380633749357194No Hit
ATATAGATGGATACGAATAAGGCGTCC461030.12360258077191251No Hit
TGATGTATTTATTAGATAAAAAATCAA458570.12294305243601483No Hit
AAGAGGTGCACAATCGACCGATCCTG442790.11871241945208585No Hit
TCCAACGGGGCCTTTCCTTCTGGCT423170.11345227882187756No Hit
AGAGATGTATTTATTAGATAAAAAAT401960.10776585768188175No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACC400150.10728059496319282No Hit
GAGATGTATTTATTAGATAAAAAATCA397880.10667200580771001No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGG397000.10643607697210433No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAG387810.10397222924572237No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTT387070.10377383454305397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)