FastQCFastQC Report
Sun 1 Oct 2023
SRR7471254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26504863
Total Bases743 Mbp
Sequences flagged as poor quality0
Sequence length20-45
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA421437715.900391562106925No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA5523132.083817599811778No Hit
ACGTTCTAGCATTCAAGGTCCCA4613431.7405975650581555No Hit
AGAGATGTATTTATTAGATAAAAAATCA3459821.3053529082568733No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG3392481.2799462498636571No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC3245921.2246507367346136No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA3054711.1525092583953367No Hit
TCCGATATAGTGTAACGGCTATCACATCACG3051321.151230247822824No Hit
ACGCGAAACTCAGGTGCTGCAATCT2991681.1287287166887074No Hit
ACGGAGTCGACCCGCAACGCGA2255170.8508514079095598No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA2049440.773231689595981No Hit
ACGTTCTAGCATTCAAGGTCCC2004570.7563027207497733No Hit
AAGAGGTGCACAATCGACCGATCCTGA1780210.6716541036261912No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT1750970.6606221658267013No Hit
ATGTATTTATTAGATAAAAAATCAATG1710230.6452514016012836No Hit
TACGCGAAACTCAGGTGCTGCAATCT1551960.5855378313028821No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCG1533870.5787126686902702No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT1508410.5691068842725201No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAG1481630.5590030780389244No Hit
ACACGGAGTCGACCCGCAACGCGA1475120.5565469249925948No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC1379160.5203422481376343No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG1279450.48272273657856674No Hit
GTCTAGTCGGTTATGGCATCTGCT1174730.4432130058548124No Hit
ATGTATTTATTAGATAAAAAATCA1149160.4335657196190752No Hit
TCCAACGGGGCCTTTCCTTCTGGC1065210.40189228670980115No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA972250.36681947761812617No Hit
AGAGATGTATTTATTAGATAAAAAATC721610.27225569888816253No Hit
ATGTACACGGAGTCGACCCGCAACGC687000.25919771779239154No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG656170.24756589007836036No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCA627250.2366546848402876No Hit
TGATGTTGACGCAATGTGATTTCTGCCCA618960.2335269569210752No Hit
AAGAGATGTATTTATTAGATAAAAAATCA602300.2272413179422961No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC565690.21342875833766806No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCC560120.21132725719050122No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGA537330.2027288350820753No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT516570.19489631016013928No Hit
AAAACCCTGGTTCGATTCTAGGAGATGGC502910.18974253894464574No Hit
ATATATGATGATATCCTATAACAACAACA501220.18910492010466154No Hit
AGCGTGTTACCTATACTCTACCGTCAGGGT496560.18734675217902466No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG484720.18287964740659102No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGC455870.17199485241632828No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC452250.17062906531529703No Hit
TACACGGAGTCGACCCGCAACGCGA442250.1668561727710119No Hit
TATACGCGAAACTCAGGTGCTGCAATCT439660.16587899360204203No Hit
ATACGCGAAACTCAGGTGCTGCAATCT424060.15999328123295714No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTC404870.15275310044047388No Hit
ACGTTCTAGCATTCAAGGTCC404710.1526927341597653No Hit
AAACCCTGGTTCGATTCTAGGAGATGGC401540.1514967272232269No Hit
AGAGATGTATTTATTAGATAAAAAATCAA399240.15062896193804134No Hit
TTGTCCGCGCGAGTTCGAACCTCGCATCCT398640.15040258838538423No Hit
ATGTACACGGAGTCGACCCGCAACGCG393550.14848218608034305No Hit
ATCAAACAGTTATCCCTGTCTGAATGGGTA391090.14755405451444892No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT386990.14600716857129198No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGT384520.14507526411285354No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA374610.14133632760146694No Hit
ATATATGATGATATCCTATAACAACAAC361210.1362806515921248No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT360230.13591090812278486No Hit
ATATATGATGATATCCTATAACAACA343410.1295649028632972No Hit
TTGTCCGCGCGAGTTCGAACCTCGCATCCTT341450.1288254159246173No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC341000.12865563576012448No Hit
GTCTAGTGGTATGATTCTCGCT335800.12669373163709619No Hit
ATGTATTTATTAGATAAAAAATC330270.12460732206010648No Hit
ACTATGCCGACTAGGGATCGGGTGGTG327780.12366787181657948No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG325310.12273596735814105No Hit
AAGCGGTGGCTCAATGGTAGAGCTTTCGACT323070.12189083942822115No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT316050.11924226886213295No Hit
TGATGTATTTATTAGATAAAAAATCAATG314960.11883102357480588No Hit
TTGTACACGGAGTCGACCCGCAACGCGA311400.11748787382904036No Hit
TAGAGATGTATTTATTAGATAAAAAATCA309870.11691062126976473No Hit
TTTGGAAGAGATGTATTTATTAGATAAA302760.11422809467077795No Hit
TGATGTATTTATTAGATAAAAAATCA297390.11220205137449683No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTG296800.111979450714384No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG291080.10982135617905288No Hit
TTACGCGAAACTCAGGTGCTGCAATCT285950.10788586230383458No Hit
AAGCGGTGGCTCAATGGTAGAGCTTTCGA285610.10775758395732889No Hit
TCGAAATGAAGATAAAAATTTCCATCGA285600.1077538110647846No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCA283820.10708223619190184No Hit
ACCTGCGCAGGTTCGAATCCTGTCTGTGACG283640.10701432412610472No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC282370.10653516677298049No Hit
CTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA278190.10495809768946927No Hit
ATTACCCAATCCTAATTCAGGGAGGTAG277610.10473926992190075No Hit
TCGAAATGAAGATAAAAATTTCCATCG272440.1027886844765053No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC271000.10224538795012826No Hit
ACGGGCTTGGCAGAATCAGCGGGGA268490.10129839192151266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)