FastQCFastQC Report
Sun 1 Oct 2023
SRR7471255.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471255.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63565410
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC46557167.32429162338448No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA38370456.036372612085724No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG33800405.317420276216263No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT32230035.070372392784063No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT20957543.2970038264521535No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT20550503.232968999963974No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA14839072.334456743061989No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT11933871.8774157202793154No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA11790011.8547839147108467No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA8189901.2884208565633417No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT7409241.1656087799952837No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG6980681.0981884644494546No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG6632891.043474745148344No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT6393911.0058788262358411No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTC6157960.9687595816655631No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5661900.8907202832483894Illumina Single End Adapter 1 (95% over 22bp)
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT5601330.881191515951836No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT5086960.8002717201068945No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT4761190.7490221489958139No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA4612150.7255754348158849No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC4278910.673150696267042No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC3827310.6021057679011274No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC3642050.5729609861715672No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT3057100.48093766719981823No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG2640550.4154067440137647No Hit
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTATG2572630.40472168747122056No Hit
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG2477300.38972453729158674No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT2187470.3441289846160042No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG2171110.3415552578045198No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT2068110.3253514765341717No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTATGCCGT1915770.30138561208053244No Hit
AGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGTCTTCTG1743970.27435833419465083No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG1601480.2519420546489042No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT1595060.2509320713891407No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTAT1536290.24168647696915666No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT1367250.21509339749401446No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG1313620.20665641895490014No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGCTGTAGGCACCATCAATTCGTA1279670.2013154638662757No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1269290.19968250027806003No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC1251810.19693257701004366No Hit
TCGTATGGCGCAGTGGTAGCGCAGCAGACTGTAGGCACCATCAATTCGTA1237990.19475843859105132No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGTAT1234170.1941574828196656No Hit
ATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCGTCTT1233560.1940615186781616No Hit
TGCTTGGTGGGGCTTGCTCTGCTAGGCGCTGTAGGCACCATCAATTCGTA1182640.1860508726365487No Hit
ATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGTATGC1131800.1780528120561167No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC1129360.17766895549010067No Hit
ATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTATGCCG1122510.17659132537649014No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATTCG1120880.17633489660493026No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG1105580.17392792715409214No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT1075930.16926344060393853No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTATGCC1058310.16649149277885567No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT1026580.16149978423799988No Hit
ATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCCGTCT1010260.15893235015710588No Hit
GTCTAGTCGGTTATGGCATCTGCCTGTAGGCACCATCAATTCGTATGCCG961120.15120173062676698No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT955810.15036637064088787No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATTCG953470.14999824590134792No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT948450.1492085082122494No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGTATG936060.1472593349118648No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC896800.14108301983736124No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATG868310.13660102247432998No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATT865170.13610704312298152No Hit
CCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTCG849160.13358837770416332No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCCTGTAGGCACCATCAATTCGT838680.1319396822894716No Hit
AGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTCGTA822610.12941157777476778No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC797030.12538737656219004No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT793990.12490912903731761No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATTCGTATGC740990.11657126100500256No Hit
AGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGT730030.11484705282322571No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATT715880.11262099937686236No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG707850.11135773371083425No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT702590.11053023963819317No Hit
AAGAGGTGCACAATCGACCGATCCTGATCTGTAGGCACCATCAATTCGTA694550.10926540078951745No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCC693680.10912853389917568No Hit
TGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTA668870.10522546775046365No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGTA659240.10371049286081847No Hit
TTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTAT644760.1014325243870841No Hit
GTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATTCGTATGCC636410.10011891687633258No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)