FastQCFastQC Report
Sun 1 Oct 2023
SRR7471256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44644466
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGNACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA32352467.246689880891396No Hit
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA24861565.56878874976352Illumina Single End Adapter 1 (95% over 22bp)
ACGNTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG3380560.7572181510693845No Hit
ACGNGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC2195920.49186835385151656No Hit
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC1795570.4021931856011Illumina Single End Adapter 1 (95% over 22bp)
ACGNTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT1461070.32726788578902477No Hit
AAGNGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1034220.23165693145484148No Hit
ACCNGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1016490.22768555457690995Illumina Single End Adapter 1 (95% over 22bp)
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT998830.22372985713391666Illumina Single End Adapter 1 (95% over 22bp)
ACGNGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT913240.20455838804298837No Hit
ACANGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTATGCC871010.19509920893666866No Hit
ACCNTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG834610.18694590276877765No Hit
TTGNACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA828370.18554819313999635No Hit
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG796710.17845660870935268Illumina Single End Adapter 1 (95% over 22bp)
AGANATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA777550.17416492337482545No Hit
ACGNAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTATGCCGT738830.16549195593469523No Hit
TCCNTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT646910.1449026179414936No Hit
TCCNTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTCG578230.12951885234779156No Hit
ATANACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA543230.12167913487866558No Hit
ATGNACACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAATTCGTATG500960.11221099609523832No Hit
CCTNTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA474970.10638944589459307Illumina Single End Adapter 1 (95% over 22bp)
ACTNTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA453460.10157137952999595Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)