FastQCFastQC Report
Sun 1 Oct 2023
SRR7471257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77188851
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length20-45
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGAAACTCAGGTGCTGCAATCT60576907.847882073021141No Hit
ATGTATTTATTAGATAAAAAATCAATG51802406.711124641562549No Hit
AGAGATGTATTTATTAGATAAAAAATCA38188874.947459316371998No Hit
ACGTTCTAGCATTCAAGGTCCCA24057673.1167286063112924No Hit
TGATGTATTTATTAGATAAAAAATCAATG15378481.9923188129850513No Hit
AAGAGGTGCACAATCGACCGATCCTGA13428301.7396683362989818No Hit
ACGTTCTAGCATTCAAGGTCCC11508031.4908927715480569No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT11258281.4585370625610168No Hit
AGAGATGTATTTATTAGATAAAAAATC10837401.404011053357952No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA10311971.3359403419543063No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA9061341.1739182385290332No Hit
ACTATGCCGACTAGGGATCGGGTGGTG8778871.1373235753956228No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA6794890.8802942279837797No Hit
GATGTATTTATTAGATAAAAAATCAATG6520490.8447450526242449No Hit
TGATGTATTTATTAGATAAAAAATCA6443060.8347138111953499No Hit
TACGCGAAACTCAGGTGCTGCAATCT5898050.7641064640280758No Hit
ATTTATTAGATAAAAAATCAATG5838340.7563708909205036No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA5710390.7397946628328487No Hit
ATGTACACGGAGTCGACCCGCAACGCGA5239270.6787599416397583No Hit
ATGTATTTATTAGATAAAAAATCA5124360.6638730766960114No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC4905820.6355606977489534No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC4750350.6154191879342783No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT4517040.5851933202114902No Hit
ATGTATTTATTAGATAAAAAATCAAT4431340.5740906805310524No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGA3762400.48742790587723606No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT3509080.4546096948638347No Hit
ATTTATTAGATAAAAAATCA3158390.4091769677981086No Hit
CAAGAGGTGCACAATCGACCGATCCTGA3141400.40697587272027147No Hit
AGATGTATTTATTAGATAAAAAATCAATG3084140.3995577029641237No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC2944620.3814825537434156No Hit
TCGCGAAACTCAGGTGCTGCAATCT2860700.3706105173142168No Hit
GATGTATTTATTAGATAAAAAATCA2447570.3170885391207598No Hit
ACGTTCTAGCATTCAAGGTCC2418690.31334706614560176No Hit
AGAGATGTATTTATTAGATAAAAAATCAA2375820.30779315525761614No Hit
TTGTATTTATTAGATAAAAAATCAATG2299680.2979290364096753No Hit
ATTACCCAATCCTAATTCAGGGAGGTAG2178400.28221692275222493No Hit
ATGTATTTATTAGATAAAAAATCAA2142760.2775996756319122No Hit
TTGGAAGAGATGTATTTATTAGATA2051720.2658052261977575No Hit
AGATGTATTTATTAGATAAAAAATCA1922010.24900098590663047No Hit
CGATGTATTTATTAGATAAAAAATCAATG1829670.23703811836763836No Hit
TGAGATGTATTTATTAGATAAAAAATCA1823210.23620120993898458No Hit
TTGGAAGAGATGTATTTATTAGATAA1671800.21658568282095558No Hit
TATGTATTTATTAGATAAAAAATCAATG1668400.2161452047006115No Hit
GGATGTATTTATTAGATAAAAAATCAATG1577770.2044038717456748No Hit
TCCAGATCGGGGTTCAATTCCCCGTCGCGGA1528780.1980571002410698No Hit
TGCTTGGTGGGGCTTGCTCTGCTAGGCG1502020.19459027832918513No Hit
TTTGGAAGAGATGTATTTATTAGATA1471160.19059229162512084No Hit
AATGTATTTATTAGATAAAAAATCAATG1460840.18925531097748818No Hit
CCAGATCGGGGTTCAATTCCCCGTCGCGGA1390280.18011409445646498No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1379170.1786747674220465No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGG1305400.1691176877344631No Hit
AAGAGGTGCACAATCGACCGATCCTGAT1276470.16536973713988826No Hit
AGAGATGTATTTATTAGATAAAAAA1262730.16358968732414478No Hit
TCGTATGGCGCAGTGGTAGCGCAGCAGA1256050.16272427737005699No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC1216750.15763286850843264No Hit
ATTTCCACGTTCTAGCATTCAAGGTCCCA1194370.15473348605746184No Hit
TTTCCACGTTCTAGCATTCAAGGTCC1191350.15434223784468565No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT1161520.1504776901006079No Hit
ACCAACGGGGCCTTTCCTTCTGGCTAACCT1138080.14744098211800044No Hit
ATGTATTTATTAGATAAAAAATCAATGT1132650.14673751264933327No Hit
AACGGGGCCTTTCCTTCTGGCTAACCT1129330.1463073987200561No Hit
ATTCCACGTTCTAGCATTCAAGGTCCCA1124720.14571016220982483No Hit
TGATGTATTTATTAGATAAAAAATCAAT1123870.1456000426797388No Hit
GCCAACGGGGCCTTTCCTTCTGGCTAACCT1106820.14339117445860153No Hit
TCGTTCTAGCATTCAAGGTCCCA1076330.13944112213822177No Hit
ATATAGATGGATACGAATAAGGCGTCC1076130.13941521166055446No Hit
ATGAAGAGCTTTTAGTGGGCCATTTTTG1072370.13892809468040923No Hit
GAGATGTATTTATTAGATAAAAAATCA1064050.13785021880944956No Hit
TAGAGATGTATTTATTAGATAAAAAATCA1031830.13367604085724763No Hit
AAGAGATGTATTTATTAGATAAAAAATCA998580.1293684239450591No Hit
TCCAACGGGGCCTTTCCTTCTGGC975840.12642240263428717No Hit
GTCTAGTCGGTTATGGCATCTGC964190.12491311731016698No Hit
ATGTATTTATTAGATAAAAAATC953430.12351913361166628No Hit
ACCAGATCGGGGTTCAATTCCCCGTCGCGGA914150.11843031579780869No Hit
TGGAAGAGATGTATTTATTAGATAA895390.115999912992616No Hit
GTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA880790.11410844812290313No Hit
TTTGGAAGAGATGTATTTATTAGATAA855720.11086056974730717No Hit
CTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA855170.1107893159337221No Hit
GTTCCACGTTCTAGCATTCAAGGTCCCA850060.11012730322932258No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCT849000.1099899776976859No Hit
TTCCACGTTCTAGCATTCAAGGTCCCA840590.10890044211177595No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGG827120.10715537144088336No Hit
ATGCCAGATCGGGGTTCAATTCCCCGTCGCGGA808680.10476642539995835No Hit
TTGGAAGAGATGTATTTATTAGATAAA795820.103100381685951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)