FastQCFastQC Report
Sun 1 Oct 2023
SRR7471258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15900492
Total Bases428.6 Mbp
Sequences flagged as poor quality0
Sequence length20-45
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA9728576.118408159948761No Hit
ACGCGAAACTCAGGTGCTGCAATCT5863373.687539983039519No Hit
ATGTATTTATTAGATAAAAAATCAATG2481571.5606875560831703No Hit
ACGTTCTAGCATTCAAGGTCCCA2063811.297953547600917No Hit
AGAGATGTATTTATTAGATAAAAAATCA1362900.857143288396359No Hit
ACGTTCTAGCATTCAAGGTCCC1193700.7507314867992764No Hit
ACTATGCCGACTAGGGATCGGGTGGTG1147670.721782697038557No Hit
AAGAGGTGCACAATCGACCGATCCTGA1009410.6348294128257164No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA838280.5272038123097071No Hit
TACGCGAAACTCAGGTGCTGCAATCT761840.4791298281839329No Hit
TGATGTATTTATTAGATAAAAAATCAATG688930.433275901148216No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA684730.4306344734489977No Hit
TGATGTATTTATTAGATAAAAAATCA604610.3802460955296226No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT569320.3580518137426188No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA557350.35052374479984644No Hit
ATCGGGCGTTCGACTCGCCCCCGGGAGA545780.34324724039985677No Hit
AAACATAGCATTGCGATGGTCA503280.3165185077291948No Hit
AGAGATGTATTTATTAGATAAAAAATC500080.3145059913869332No Hit
TTGTACACGGAGTCGACCCGCAACGCGA479630.30164475413716757No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT445660.2802806353413467No Hit
ATGTATTTATTAGATAAAAAATCA434930.27353241648120075No Hit
AAAACATAGCATTGCGATGGTCA409380.2574637313109557No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC392060.24657098660846472No Hit
TCGCGAAACTCAGGTGCTGCAATCT342680.21551534380194023No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA312560.19657253373040282No Hit
TCCAACGGGGCCTTTCCTTCTGGC304230.19133370212695305No Hit
ACGGGCTTGGCAGAATCAGCGGGGA300850.18920798174043924No Hit
ATGTATTTATTAGATAAAAAATCAAT300080.18872371999558252No Hit
ACGTTCTAGCATTCAAGGTCC282540.17769261479456108No Hit
ACACGGAGTCGACCCGCAACGCGA280200.17622096221928227No Hit
ACGGAGTCGACCCGCAACGCGA269060.16921488970278403No Hit
ATATATGATGATATCCTATAACAACA251190.15797624375396685No Hit
AAACATAGCATTGCGATGGTCAG238900.15024692317696836No Hit
ATCTGGTTGATCCTGCCAGTAGTC229970.14463074475934456No Hit
ATGCCGACTAGGGATCGGGTGGTGTT226250.14229119451146544No Hit
AAAACATAGCATTGCGATGGTCAG220870.13890765141103809No Hit
ATTTATTAGATAAAAAATCAATG219890.1382913182812205No Hit
TCCAGATCGGGGTTCAATTCCCCGTCGCGGA219390.1379768626027421No Hit
ATGCAAGAGTTCGAATCTCTTAGCAACCAC210610.13245502088866182No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA203120.12774447482505571No Hit
TCGGGCTTGGCAGAATCAGCGGGGA186640.11738001566240844No Hit
TATACGCGAAACTCAGGTGCTGCAATCT184570.11607816915350795No Hit
ATGTACACGGAGTCGACCCGCAACGC182430.11473229884962051No Hit
ACGTGAAATTGTTGAAAGGGAAGGGC180750.11367572776993316No Hit
TTACGCGAAACTCAGGTGCTGCAATCT180490.1135122108171244No Hit
ACTTATGTTGCCTTCCTTCTGAAA177330.11152485092914106No Hit
TTTGGAAGAGATGTATTTATTAGATA173900.1093676849747794No Hit
CCAGATCGGGGTTCAATTCCCCGTCGCGGA168140.1057451555587085No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC166400.10465084979760374No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT166230.1045439348669211No Hit
ATGCCGACTAGGGATCGGGTGGTG163900.10307857140521186No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG161830.1017767248963114No Hit
TAGAGATGTATTTATTAGATAAAAAATCA159470.10029249409389344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)