FastQCFastQC Report
Thu 5 Oct 2023
SRR7471259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174082944
Total Bases8.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT169669349.746465454995981No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA110345256.338659461089996No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC97214175.584359258078724No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT51587232.9633707251642067No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG39705292.280825972244587No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT34842532.001490163217828No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG26412551.5172393913558815No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT25377551.4577849740408801No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA25208481.4480729370018006No Hit
GATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTA24831901.4264407201201745No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG21058051.2096561280581284No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT19031071.093218529208697No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT18877041.0843704481468328No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT18700981.0742568783763216No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA18184201.0445710293134747Illumina Single End Adapter 1 (95% over 22bp)
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG17296250.9935637347677209No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC16081700.9237952685358998No Hit
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTATG15629900.8978421228905689No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT14821760.8514194245244381No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTC14383730.8262572811268634No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG13823990.7941036429163331No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA13392700.769328671279824No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT12051470.6922832141441726No Hit
ATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCCGTCT9160210.5261980174232348No Hit
GATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGC9151610.5257040000426464No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATT9060460.5204679902472238No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA8877490.5099574832557979No Hit
ATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCGTCTT8431220.4843220022749615No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA7914560.45464304647789044No Hit
AGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGT7001600.4021990804567276No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT6751490.38783179126382417No Hit
ATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGTATGC6697620.38473728936937096No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGCTGTAGGCACCATCAATTCGTA6559520.3768042893392244No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC6383010.3666648698220545No Hit
AGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGTCTTCTG5893000.33851679346599284No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT5866200.33697729744276383No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG5368790.3084041363638703No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA4810470.2763320684650186No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG4809870.27629760213614035No Hit
GAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTAT4253800.24435478297058214No Hit
AGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT4218950.24235286370156975No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC4095810.23527922413812122No Hit
TGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTA4065770.23355360993894958No Hit
AGAGATGTATTTATTAGATAAAAAACTGTAGGCACCATCAATTCGTATGC3735490.2145810447690958No Hit
ATGTATTTATTAGATAAAAAATCAATGTCTGTAGGCACCATCAATTCGTA3697930.21242345258131665No Hit
TGCTTGGTGGGGCTTGCTCTGCTAGGCGCTGTAGGCACCATCAATTCGTA3635160.20881770014183587No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT3590790.20626891512128837No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTCTGTAGGCACCATCAATTCGT3571690.2051717369853304No Hit
AGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTG3532050.20289466152410657No Hit
AGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG3432020.19714855006128573No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT3391570.19482494505607625No Hit
ATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTATGCCG3259020.1872107585680536No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGCTGTAGGCACCATCAATTCGT3239370.18608198629729056No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT3230840.18559198998840462No Hit
TCGTATGGCGCAGTGGTAGCGCAGCAGACTGTAGGCACCATCAATTCGTA2998160.17222594764941476No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATG2915650.16748625298983916No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT2874870.1651436915037466No Hit
GTCTAGTCGGTTATGGCATCTGCCTGTAGGCACCATCAATTCGTATGCCG2819590.16196819373642946No Hit
TTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTAT2608220.1498262805114325No Hit
ATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTATGCCGTCTT2576720.1480167982453238No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT2459140.1412625466628138No Hit
CGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT2452940.14090639459773843No Hit
TGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATGC2426270.13937436627909972No Hit
CCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTCG2413780.13865689219961722No Hit
ATGAAGAGCTTTTAGTGGGCCATTTTTGCTGTAGGCACCATCAATTCGTA2407380.1382892513582491No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC2400060.1378687621459343No Hit
ATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCCGTCTTCTG2346600.13479781224288118No Hit
TGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGTAT2254130.12948597652392643No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGTAT2147480.12335958656581544No Hit
AGAGATGTATTTATTAGATAAAAAATCTGTAGGCACCATCAATTCGTATG2123610.12198840111527526No Hit
AAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT2096860.12045177728611943No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG2094290.12030414651075755No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTAT1984810.11401519036810406No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATTCG1966600.11296913728664883No Hit
AAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT1957640.11245444010873344Illumina Single End Adapter 1 (95% over 21bp)
GATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTAT1916000.1100624768845821No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC1846010.10604197962093288No Hit
AAGAGGTGCACAATCGACCGATCCTGATCTGTAGGCACCATCAATTCGTA1778550.102166815377387No Hit
AGAGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCG1769550.10164982044421307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)