FastQCFastQC Report
Sun 1 Oct 2023
SRR7471260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9394316
Total Bases479.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8870939.442869496831914Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA3979124.235667610074007No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC1808521.9251215309342373No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT1599841.7029872105643453No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA730940.778066226428832No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG682730.7267479612139937No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT519060.5525255910063064No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA420640.44776011366873325Illumina Single End Adapter 1 (95% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT408540.43487998487596113No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT401790.42769478906181146No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT340590.36254901368018705No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC306860.32664432407851723No Hit
AAGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC268000.28527888565809373No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT261730.278604637101839No Hit
TTGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA257960.27459157218045466No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG246070.2619349828130116No Hit
ACGAGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC229800.24461599971727588No Hit
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTATG210190.2237416752853534No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG196340.20899871794817204No Hit
ATCGGGCGTTCGACTCGCCCCCGGGAGACTGTAGGCACCATCAATTCGTA178620.19013624834421156No Hit
CCGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA170780.18179077646525835Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA163750.17430752808400313Illumina Single End Adapter 1 (95% over 22bp)
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA161650.1720721338307121Illumina Single End Adapter 1 (95% over 22bp)
AGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG159890.17019866055176341No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA158980.16922998970867065No Hit
AAAACATAGCATTGCGATGGTCACTGTAGGCACCATCAATTCGTATGCCG153910.16383310929715372No Hit
CAGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA153330.16321571469386384Illumina Single End Adapter 1 (95% over 22bp)
AGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT145590.15497669016030546No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG142130.15129361200964497No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT141220.1503249411665522No Hit
AAGAGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC139800.1488133888619459No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT134200.14285233751983648No Hit
CTGAAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA133180.14176657459680939Illumina Single End Adapter 1 (95% over 22bp)
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA130100.13848799635864922Illumina Single End Adapter 1 (95% over 22bp)
AAACATAGCATTGCGATGGTCACTGTAGGCACCATCAATTCGTATGCCGT129130.13745545710831952No Hit
AAGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG124610.13264403709647407No Hit
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG121470.129301590451077No Hit
ACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA120170.12791777496094447Illumina Single End Adapter 1 (95% over 22bp)
ACGGGCTTGGCAGAATCAGCGGGGACTGTAGGCACCATCAATTCGTATGC114350.12172253945896647No Hit
ACGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA108240.1152186066553435No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA106960.11385608063428992No Hit
ATATATGATGATATCCTATAACAACACTGTAGGCACCATCAATTCGTATG106740.11362189647442134No Hit
ATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGTATGC100190.10664959535106122No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTC94620.10072047821257024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)