FastQCFastQC Report
Sun 1 Oct 2023
SRR7471261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15935239
Total Bases478.8 Mbp
Sequences flagged as poor quality0
Sequence length15-45
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACGTTCTAGCATTCAAGGTCCCA5884663.6928595799535855No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT5746353.6060645215299254No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA3311392.0780297051082823No Hit
TTGCCTTGTTGTAGACGGCCTTGGTAGGTC3057481.9186910218290418No Hit
ATGTACACGGAGTCGACCCGCAACGCGA2406851.5103946668135948No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGG2240121.4057649213795915No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC2163541.3577079076128071No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC2070021.299020366120646No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCT2009661.2611420512739093No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCA1952521.2252844152510043No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCA1824861.1451726579061663No Hit
TCCAACGGGGCCTTTCCTTCTGGCT1779431.1166635153699296No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC1756821.1024748358025882No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCT1685661.0578190888759185No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT1486670.9329449028031521No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTT1400660.8789701867665743No Hit
TTGCCTTGTTGTAGACGGCCTTGGTAGGTCT1388450.8713079232762057No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT1380130.8660867904146277No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG1372370.8612170799571942No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGT1362150.8548036210815538No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGTAGGTC1310920.82265474650239No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCA1102140.6916369437571661No Hit
TCCAACGGGGCCTTTCCTTCTGGC978620.6141232020429691No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTG927080.5817797900615108No Hit
TTTCCAACGGGGCCTTTCCTTCTGGC919810.577217574207704No Hit
TTGGAAGAGATGTATTTATTAGATAAA834210.5235001495741608No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAG831660.5218999225552877No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGA825520.5180468269098443No Hit
AGAGATGTATTTATTAGATAAAAAATCA814830.5113384242307254No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG794500.49858053588025886No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTT724020.4543515161586218No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATC708660.44471250164493925No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT681100.427417499040962No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACC673070.4223783527815303No Hit
TTGGAAGAGATGTATTTATTAGATAA594670.3731792162012757No Hit
TTGCCTTGTTGTAGACGGCCTTGGTAGGT590490.37055609897033864No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA558020.35017987493002145No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCC555840.3488118377138868No Hit
TTCCAACGGGGCCTTTCCTTCTGGCT538680.3380432511868821No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCC524080.3288811670788245No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACC513370.32216021359955754No Hit
ACGCGAAACTCAGGTGCTGCAATCT497580.3122513568826925No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGG480750.3016898585581302No Hit
TTGGAAGAGATGTATTTATTAGATAAAA460360.2888943178072196No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG455240.2856813129693254No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGTAGGTCT442220.2775107420729617No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC428590.2689573717720833No Hit
TAGAGATGTATTTATTAGATAAAAAATCA424130.26615854333907385No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGA420880.2641190383150199No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT398230.2499052571473826No Hit
TTGCCTTGTTGTAGACGGCCTTGGTA383220.2404858816362905No Hit
TTTGGAAGAGATGTATTTATTAGATAAA372720.23389671155857783No Hit
AAGAGGTGCACAATCGACCGATCCTGA372420.23370844955635747No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG366250.229836527710692No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATC352440.22117020020848135No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTG347130.21783796276918094No Hit
TTGCCTTGTTGTAGACGGCCTTGGT340960.21396604092351548No Hit
AGAGATGTATTTATTAGATAAAAAATC331890.20827425305638653No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTT331670.2081361942547583No Hit
TACGCGAAACTCAGGTGCTGCAATCT329390.20670540303788354No Hit
TTCCAACGGGGCCTTTCCTTCTGGC327530.20553817862411727No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA321450.20172273537911795No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC298520.18733324300940826No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT284610.17860416150645747No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC281370.17657093188247758No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC281220.17647680088136738No Hit
TTGGAAGAGATGTATTTATTAGATA277870.17437454185657333No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGTAGGT266630.16732099217338378No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGT265020.16631065276146784No Hit
ACTACTTGCGTGCCTTGTTGTAGACGGCCTTGG264460.16595923035732316No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGGTAGGTC261000.16378794193171498No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC257930.16186139410899328No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCAT239070.15002598956940652No Hit
TCCACGTTCTAGCATTCAAGGTCCCA238970.1499632355686664No Hit
TTTGGAAGAGATGTATTTATTAGATAA234740.1473087413373593No Hit
TTTGGAAGAGATGTATTTATTAGATAAAA231870.14550770151611783No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT230360.14456011610494202No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC224660.14098313806275511No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGG219160.1375316680220485No Hit
ATGCCTTGTTGTAGACGGCCTTGGTAGGTC218040.13682882321375914No Hit
ACTATGCCGACTAGGGATCGGGTGGTG217650.13658408261087268No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGA211750.13288159656720555No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCA209180.13126881874818444No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGTA206980.1298882307319018No Hit
TCCAACGGGGCCTTTCCTTCTGGCTA206020.12928579232479662No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA201830.12665639969378556No Hit
TTGGAAGAGATGTATTTATTAGATAAAAA195030.12238912764345737No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGC192070.12053160922154979No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCAA189660.11901923780371287No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAG188240.11812813099320318No Hit
AACGGGGCCTTTCCTTCTGGCTAACCT187310.11754451878632005No Hit
TTGCCTTGTTGTAGACGGCCTTGG186770.1172056471823234No Hit
TCGATGAAACCCTGGTTCGATTCTAGGAGATGGC186040.11674754297692053No Hit
ACGTTCTAGCATTCAAGGTCCCA185400.11634591737218374No Hit
TAGAGATGTATTTATTAGATAAAAAATC184310.11566189876411642No Hit
ACTTGCGTGCCTTGTTGTAGACGGCCTTGG182250.11436916634886994No Hit
AAGAGATGTATTTATTAGATAAAAAATCA181560.11393616374376311No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATG180030.11297602753243927No Hit
AAGAGGTGCACAATCGACCGATCCTGAT178050.11173349831778488No Hit
TACCATACGCGAAACTCAGGTGCTGCAATCT176660.1108612177074972No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCA173060.10860207368085285No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG171910.10788040267234147No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAAC171810.10781764867160136No Hit
TCCGATATAGTGTAACGGCTATCACATCACG168340.10564008484591916No Hit
TATACGCGAAACTCAGGTGCTGCAATCT167520.10512550203985017No Hit
AGAGATGTATTTATTAGATAAAAAATCAAT166810.1046799486345953No Hit
TTACGCGAAACTCAGGTGCTGCAATCT165800.10404613322712009No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT164960.10351899962090308No Hit
TTTTCCAACGGGGCCTTTCCTTCTGGCTAACCT163000.10228902120639671No Hit
TTGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCC162330.1018685694014379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)