FastQCFastQC Report
Sun 1 Oct 2023
SRR7471263.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471263.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38052832
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT994068426.12337499611067No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG24791486.5150157549377665No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG13936613.6624370033746767No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATTCGTATGC9227482.4249128159502034No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8644262.271646956526127Illumina Single End Adapter 1 (95% over 22bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCC6597701.733826276057456No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG4030571.0592036881775317No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT3469610.9117875904742122No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA3438930.9037251156497367No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATGC2893730.7604506282213108No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT2333660.6132684158698096No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1922290.5051634527490622No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATGC1829660.4808209806828569No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCG1739570.4571460016431892No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTA1606980.4223023400728755No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT1592300.41844454573052536No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATGCCGT1543860.40571487557089053No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1469370.38613946000129507No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1463790.3846730776831538No Hit
ATGATTAATAGGGACGGTCGGGGGCTGTAGGCACCATCAATTCGTATGCC1417100.37240329445125137No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC1132070.2974995395874872No Hit
TCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT1105910.2906248869992121No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTATG1097190.2883333361364536No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC1021420.26842154612828817No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1005760.26430621510640784Illumina Single End Adapter 1 (95% over 22bp)
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT993420.2610633552845686No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC948370.249224551802084No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT899750.2364475789870252No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA888830.23357788455797457No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATGCCGTCT886320.23291827530734113No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATTCGTATGCCG831480.21850673295485604No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT823120.21630978740294549No Hit
GCCAAGGACGTTTTCATTAATCAAGAACCTGTAGGCACCATCAATTCGTA786630.20672048797839804No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT766640.2014672653010425No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA723570.19014879102822096No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT720810.18942348364505432No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT648760.17048928184898302No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGT643580.1691280165428949No Hit
ATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTATGCC639950.16817407965851267No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG622470.1635804662317906No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATTCG584690.1536521644433718No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC576530.15150777739748778No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACATCAATTCGTATG547050.1437606536091716No Hit
TTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATTCGTATGCC545440.1433375576356577No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA516550.1357454814401199Illumina Single End Adapter 1 (95% over 22bp)
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG505790.1329178338158905No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATG497380.13070774863747328No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT485520.12759102923009777No Hit
AAGAGGTGCACAATCGACCGATCCTGATCTGTAGGCACCATCAATTCGTA480720.12632962508546014No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATTCGTATG480440.12625604317702294No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATTCGTATGC479900.12611413521075118No Hit
AAGAGGTGCACAATCGACCGATCCTGCCTGTAGGCACCATCAATTCGTAT472060.12405384177450972No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTA455740.11976506768274173No Hit
CTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTATG446450.11732372507780763No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG439700.11554987549941091No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT438730.11529496674518207No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT419920.11035183925338328No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATTCGTAT395020.10380830525307551No Hit
AATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTAT393680.10345616326269752No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA391140.10278867023616009No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT381880.10035521140712995No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATTCG380560.1000083252673546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)