FastQCFastQC Report
Sun 1 Oct 2023
SRR7471265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43882857
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCA19297904.397594258732972No Hit
ACGTTCTAGCATTCAAGGTCCCA9195782.095529012616476No Hit
AAGAGGTGCACAATCGACCGATCCTGA6287911.4328852836541612No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC5862721.335993233075048No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT5820361.3263402608449126No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA5117091.1660795011591885No Hit
ACGCGAAACTCAGGTGCTGCAATCT4920481.12127612839793No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT4745171.081326587282136No Hit
TACGCGAAACTCAGGTGCTGCAATCT4215210.9605596098722561No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA3420410.7794410468762323No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC2828860.6446389759901002No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA2594140.5911511185335996No Hit
ACGTTCTAGCATTCAAGGTCCC2587040.589533174651778No Hit
ATGTACACGGAGTCGACCCGCAACGCGA2300050.5241340599131912No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG1993180.4542047023055039No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC1899920.43295266759864787No Hit
TTACGCGAAACTCAGGTGCTGCAATCT1831000.4172472179739801No Hit
TTTTGTGCCCTCTGCTCCTTGTGGGTAGGGGA1818820.4144716466386863No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT1707790.38917019463887687No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG1663270.37902500286159585No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC1643990.3746314876444804No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1413580.32212579048807144No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCA1382940.3151435650600416No Hit
ATCAGCTTGCGTTGATTACGTCCCTGCCCT1333250.30382023668149044No Hit
TTTGTGCCCTCTGCTCCTTGTGGGTAGGGGA1298560.29591509960256235No Hit
ATCAGCTTGCGTTGATTACGTCCCTGCCC1227940.2798222549639373No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA1171310.2669174434107606No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGA1149830.26202259346970047No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGC1020060.23245068113956208No Hit
TCCACGTTCTAGCATTCAAGGTCCCA1008780.22988020128224557No Hit
AAGAGGTGCACAATCGACCGATCCTGAT933600.21274822648853514No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGA910990.20759587280290343No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAG875150.1994286743910042No Hit
ACTATGCCGACTAGGGATCGGGTGGTG869710.19818901034634093No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTT793030.1807152164226682No Hit
TCCGATATAGTGTAACGGCTATCACATCACG792650.18062862224307774No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG792450.18058304635908276No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT783170.178468325341716No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAG769210.1752871286388669No Hit
CAAGAGGTGCACAATCGACCGATCCTGA741130.16888827452597263No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC731910.16678722627380438No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC674200.15363630494705482No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT659390.15026141073722707No Hit
TCACGTTCTAGCATTCAAGGTCCCA651030.1483563387862372No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA638030.14539390632656393No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGA628100.1431310636862135No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAA616380.1404603168841081No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGG616350.14045348050150883No Hit
AGAGATGTATTTATTAGATAAAAAATCA570680.13004622739125668No Hit
TATACGCGAAACTCAGGTGCTGCAATCT529540.12067126805349068No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT521130.11875480213150205No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGC517540.11793671501379228No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGA493070.1123605056070073No Hit
ACGCGCGCTACACTGACGGAGCCAGCG486940.11096360476256137No Hit
TCTATGCCGACTAGGGATCGGGTGGTGT485200.11056709457180512No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA484200.11033921515183023No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTT478390.10901523572177628No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT462950.10549677747736434No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCG458360.1044508109396797No Hit
TTGGGTACAAATGGCAGTCTGACAAGT454980.10368057850016466No Hit
ATGTATTTATTAGATAAAAAATCAATG453810.10341395957879405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)