FastQCFastQC Report
Sun 1 Oct 2023
SRR7471267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33175273
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1013253130.54241934949563No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG32367139.756402004589383No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG18813175.670841050803109No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATTCGTATGC5787831.744621664454728No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATGC5563261.6769296819350965No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA3976071.1985040786250651No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCC3589471.0819715032940347No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3185840.9603055866337559Illumina Single End Adapter 1 (95% over 22bp)
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG3075330.9269946324179457No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA2115900.6377942993867752No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATGC1913070.5766553902962607No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1766560.5324929805400547No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1705870.5141992350748704No Hit
TCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT1623790.4894579164427675No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1362960.41083610675939275No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT1354140.4081775001519957No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT1341550.4043825050060628No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATTCG1207860.36408441913952No Hit
ATGATTAATAGGGACGGTCGGGGGCTGTAGGCACCATCAATTCGTATGCC994250.29969610197329805No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTA992570.29918970071474615No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATTCGTATGCCG962720.2901920354958345No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT950340.2864603405072205No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT848750.2558381358308642No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCG845620.25489466205749084No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT704540.21236901351196114No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA665300.2005409269729295No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT599480.18070084909323883No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA586230.17670691059573196No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTATG573670.172920958329416No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC569180.17156754068007216No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGT562780.16963839302844622No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG552610.16657285683828435No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG551680.16629252757015744No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACATCAATTCGTATG519620.15662870355279368No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA506220.15258955065720184No Hit
GCCAAGGACGTTTTCATTAATCAAGAACCTGTAGGCACCATCAATTCGTA460820.1389046595034802No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATTCGTA456240.13752411321528538No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT442970.13352414613136718No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA427890.12897859197722353Illumina Single End Adapter 1 (95% over 22bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATT420900.1268716010264633No Hit
TTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATTCGTATGCC402210.12123788702507438No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT393930.11874205225078328No Hit
CTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTATG380130.11458232762696482No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC361440.10894861362557588No Hit
ATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTATGCC355650.10720333785949553No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATCGTATG349510.10535256183121688No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATT338760.1021121966351264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)