FastQCFastQC Report
Sun 1 Oct 2023
SRR7471268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26185227
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA379343614.486931887204948Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10817814.13126454851814Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA8136723.1073704268441134No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT7817902.9856147514016205No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2580980.9856626409998279Illumina Single End Adapter 1 (95% over 22bp)
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATT2268410.8662938075732549No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTCG2121350.8101323696754663No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG2013430.7689182912181742No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT1741920.6652300550993887No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC1659900.6339070499560687No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATTCGTATGC1552200.5927769883377372No Hit
TCGATGAAACCCTGGTTCGATTCTAGGAGATGGCCTGTAGGCACCATCAA1547070.5908178684110701No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACTGTAGGCACCATCA1137890.43455418583921385No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG1071750.4092956688899431No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT1007980.3849422424331093No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCTGTAGGCACCATCA944300.36062318650130476No Hit
TCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT805870.3077575000590982No Hit
GTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATTCGTATGCC763530.2915880775064505No Hit
AACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA742850.28369049464417473Illumina Single End Adapter 1 (95% over 22bp)
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTC738240.2819299599732322No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT735570.28091030106403125No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCTGTAGGCACCATCAA705590.2694610972820667No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATTCGTATGCCGT654400.24991190643487643No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAA641510.24498928346124324No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCC639920.24438207085239325No Hit
GTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA636530.2430874477429583Illumina Single End Adapter 1 (95% over 22bp)
TTCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAAT613780.23439934280500987No Hit
ACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA604530.23086681662144842Illumina Single End Adapter 1 (95% over 22bp)
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGACTGTAGGCACCATCAA577640.22059766753215465No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCG575620.21982624019260938No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC575500.2197804128259037No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA531780.20308397555614086Illumina Single End Adapter 1 (95% over 22bp)
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG530010.20240802189723234No Hit
AAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATT522540.1995552683198049No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG522340.19947888937529548No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCTGTAGGCACCATCAAT507740.1939032264261066No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG499850.19089007706520933No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG490720.18740337824835354No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGACTGTAGGCACCATCAAT463080.17684780811714942No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGTCTGTAGGCACCATCAATT455170.1738270208618012No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATT447410.17086351781483508No Hit
GCGTAGTCGGTAGCGCTCTCCCTCTGTAGGCACCATCAATTCGTATGCCG433380.16550553485749808No Hit
TCGATGAAACCCTGGTTCGATTCTAGGAGATGGCTGTAGGCACCATCAAT421880.16111374554820548No Hit
ATGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTC417730.15952888244963467No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG405980.15504161945970527No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA403400.15405633107553354No Hit
GTCTAGTGGTATGATTCTCGCTCTGTAGGCACCATCAATTCGTATGCCGT400400.152910646907892No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC391950.1496836365023683No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT391500.14951178387722208No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTATGCCGT370910.1416485715399756No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA360590.13770741800328865No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA334300.1276674057475232Illumina Single End Adapter 1 (95% over 22bp)
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT326420.12465807533385141No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT309560.1182193303117059No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATGC305060.11650080406024359No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC297570.1136404125883652No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT296450.11321269049911235No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCTGTAGGCACCATC292380.1116583789783453No Hit
CTGTAGGCACATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA291540.11133758741140568Illumina Single End Adapter 1 (95% over 22bp)
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTA287970.10997422325191224No Hit
GACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA286070.10924862327907259Illumina Single End Adapter 1 (95% over 22bp)
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATGC282150.10775159596668764No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT279100.10658681706291873No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCTGTAGGCACCATCAA276480.10558625288984509No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)