FastQCFastQC Report
Sun 1 Oct 2023
SRR7471269.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471269.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37122430
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA35368529.527533623203006No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC26852967.233621290416603No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC21876375.893032864497286No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT20533135.53119232765743No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA13730613.698736855319008No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG8902832.3982347060793163No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT6903361.8596196423563867No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC6803411.832695219574796No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6409281.7265249069093807Illumina Single End Adapter 1 (95% over 22bp)
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT6283211.6925643068085792No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT5595931.5074255645441312No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG4741641.2772978493056624No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA4358841.1741795997729674No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTC4155861.1195010671445806No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT3053250.8224811791684973No Hit
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTATG2642120.7117314249094145No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG2480920.6683075434447583No Hit
TGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTATGC2427630.653952340943198No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG2394250.6449604726845738No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG2053140.5530726302130545No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT2029230.5466317803010202No Hit
ATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGTATGC1959750.5279153331287849No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT1944850.5239015872613942No Hit
ATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTATGCCG1900590.511978876382823No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT1792080.4827485700693624No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGCTGTAGGCACCATCAATTCGTA1754420.47260376004480315No Hit
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG1568270.422458874594147No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT1457970.39274638001876494No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT1403560.3780894731298571No Hit
AGAGATGTATTTATTAGATAAAAACTGTAGGCACCATCAATTCGTATGCC1387090.3736528023623454No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC1369290.36885785763485845No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT1221990.32917834312031835No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG1171810.3156609090514818No Hit
ATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCCGTCT1158090.31196503030647504No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATTCGTATGC1088650.29325935829093086No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA1043230.28102416786832113No Hit
AGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG1019400.2746048682696688No Hit
TGCTTGGTGGGGCTTGCTCTGCTAGGCGCTGTAGGCACCATCAATTCGTA991680.26713768468281845No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT971260.2616369671920723No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT938100.25270436229524845No Hit
TAGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTA900810.24265922247008073No Hit
TGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTA892390.2403910519866291No Hit
AGAGATGTATTTATTAGATAAAAAACTGTAGGCACCATCAATTCGTATGC838850.2259685047557501No Hit
AGAGATGTATTTATTAGATAAAACTGTAGGCACCATCAATTCGTATGCCG834610.22482633814650604No Hit
TGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGTAT788370.21237025701173118No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT777590.2094663522835116No Hit
TTTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTA722930.1947421006652851No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTATGCC717540.19329014830117533No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTATGCCGT683350.1840800831195587No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT644960.173738626485389No Hit
TCGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC636120.17135731685668207No Hit
TTGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTAT620970.16727622625997274No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA593250.15980904267312243No Hit
TCGTATGGCGCAGTGGTAGCGCAGCAGACTGTAGGCACCATCAATTCGTA549960.14814762934430747No Hit
GAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTAT547330.14743916279187544No Hit
CCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTCG543280.14634817817691353No Hit
TCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT533970.14384026045708753No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTA529560.1426522994319068No Hit
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGCC528450.14235328883373205No Hit
TTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTAT523280.14096059983142267No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT463500.12485712815675051No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCCTGTAGGCACCATCAATTCGT455670.12274789123449086No Hit
TTCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGC449040.12096190901296064No Hit
TGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATGC443860.11956652622147848No Hit
AGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT423360.11404425841735039No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATT411890.11095448223621136No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATTCG411580.11087097477185627No Hit
TTTACCCAATCCTAATTCAGGGAGGTAGCTGTAGGCACCATCAATTCGTA398950.10746871904667879No Hit
ATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTATGCC390540.10520324235239989No Hit
ATGTATTTATTAGATAAAAAATCAATGTCTGTAGGCACCATCAATTCGTA382280.10297817249571217No Hit
AGAGATGTATTTATTAGATAAAAAATCTGTAGGCACCATCAATTCGTATG380870.10259834822235507No Hit
CGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG379830.10231819414838954No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA374240.10081236600082484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)