FastQCFastQC Report
Sun 1 Oct 2023
SRR7471270.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471270.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42655731
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA978942922.94985637451624No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA755472217.7109190790799Illumina Single End Adapter 1 (95% over 22bp)
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC6320491.481744621842256No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT4412461.0344354431529963No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4083260.9572594125745965Illumina Single End Adapter 1 (95% over 22bp)
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATT3197130.7495194490981764No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT2760220.6470924153192921No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2717080.6369788856742369Illumina Single End Adapter 1 (95% over 22bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTCG2216940.519728521356251No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCTGTAGGCACCATCAA2107460.49406256805211No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2029190.4757133338073611Illumina Single End Adapter 1 (95% over 22bp)
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTATGCCGT2023890.47447082784725925No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG1894550.4441489937190386No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTATGCC1756840.41186493791420425No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC1752280.4107959139183431No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCTGTAGGCACCATCAAT1660310.3892349189842743No Hit
ATGTACACGGAGTCGACCCGCAACGCCTGTAGGCACCATCAATTCGTATG1574600.36914148769364663No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCTGTAGGCACCATCAA1369740.32111511580940905No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG1350600.3166280282478338No Hit
GTCTAGTGGTATGATTCTCGCTCTGTAGGCACCATCAATTCGTATGCCGT1348830.31621307814417715No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT1204930.2824778691519787No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC1152330.2701465835856851No Hit
ACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1145880.2686344772757499Illumina Single End Adapter 1 (95% over 22bp)
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG1128000.2644427779235573No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT1062000.24897006219398746No Hit
ATGTACACGGAGTCGACCCGCAACGCGCTGTAGGCACCATCAATTCGTAT928750.2177315868763332No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTATGC886260.2077704400377056No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA820430.19233757827289374No Hit
ATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATTCGTATG803580.1883873470601172No Hit
GTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATTCGTATGCC761160.1784426106775664No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG754070.1767804659120717No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTC675120.1582718158082908No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT643710.15090820973153643No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA601960.14112054485714945Illumina Single End Adapter 1 (95% over 22bp)
TTGTCCGCGCGAGTTCGAACCTCGCATCCTCTGTAGGCACCATCAATTCG572010.1340992140071401No Hit
ATAGTGTAGTGGTTATCACTTTCGGTCTGTAGGCACCATCAATTCGTATG556230.13039982833725203No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATCA553610.12978560840980546No Hit
ATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATTCGTA538960.1263511343880146No Hit
GCGTAGTCGGTAGCGCTCTCCCTCTGTAGGCACCATCAATTCGTATGCCG505740.11856320080413109No Hit
TTGTCCGCGCGAGTTCGAACCTCGCATCCTTCTGTAGGCACCATCAATTC500650.11736992621225974No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT499080.11700186312596543No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT454590.10657184611371448No Hit
AAGCGGTGGCTCAATGGTAGAGCTTTCGACTCTGTAGGCACCATCAATTC451290.10579821032723599No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGTCTGTAGGCACCATCAATT450220.1055473647843475No Hit
GTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA446400.10465182275272694Illumina Single End Adapter 1 (95% over 22bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT444780.10427203791209204No Hit
GCTCAATGGTAGAGCTTTCGACTCTGTAGGCACCATCAATTCGTATGCCG442970.10384771040496292No Hit
AGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTCG439480.10302953195199022No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG431740.10121500438006795No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)