FastQCFastQC Report
Sun 1 Oct 2023
SRR7471271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39982269
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length20-45
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA32041438.013909865895805No Hit
ACGCGAAACTCAGGTGCTGCAATCT28814417.206797092981391No Hit
ATGTATTTATTAGATAAAAAATCA27761226.9433828280230925No Hit
ATGTATTTATTAGATAAAAAATCAATG25130336.285368646786904No Hit
AGAGATGTATTTATTAGATAAAAAATCA18319944.5820160931837055No Hit
TGATGTATTTATTAGATAAAAAATCA11982092.996850929095595No Hit
AGAGATGTATTTATTAGATAAAAAATC9320302.331108322041453No Hit
ACTATGCCGACTAGGGATCGGGTGGTG8607502.152829295405921No Hit
TGATGTATTTATTAGATAAAAAATCAATG7773261.9441768049732244No Hit
TCCAACGGGGCCTTTCCTTCTGGC6405291.6020326410189478No Hit
TACGCGAAACTCAGGTGCTGCAATCT4902131.2260759888339503No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT4593811.1489618060445743No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA4592011.1485116064823635No Hit
AAGAGGTGCACAATCGACCGATCCTGA4055831.0144071613344405No Hit
TGATGTATTTATTAGATAAAAAATC3644920.91163410460772No Hit
ATGTATTTATTAGATAAAAAATCAAT3499000.8751379267644864No Hit
ATGTATTTATTAGATAAAAAATCAA2655810.66424694406413No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT2651030.6630514141155921No Hit
ATTACCCAATCCTAATTCAGGGAGGTAG2588110.6473144383076408No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA2561750.6407215158299295No Hit
TTTGGAAGAGATGTATTTATTAGATA2531350.6331181454459225No Hit
ACGTTCTAGCATTCAAGGTCCCA2504850.6264902074467059No Hit
ATGTATTTATTAGATAAAAAATC2487160.622065746193644No Hit
ATTTATTAGATAAAAAATCAATG2287140.5720385703972928No Hit
TAGAGATGTATTTATTAGATAAAAAATCA2282650.5709155726004445No Hit
AGAGATGTATTTATTAGATAAAAA2071340.5180646451055592No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1932180.48325921672929567No Hit
TTTGGAAGAGATGTATTTATTAGATAA1904170.4762536113195577No Hit
ATTTATTAGATAAAAAATCA1697720.42461822264264193No Hit
TTGGAAGAGATGTATTTATTAGATA1671450.41804781014304115No Hit
TTGGAAGAGATGTATTTATTAGATAA1495680.37408582289314296No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC1474590.3688109846892381No Hit
AGATGTATTTATTAGATAAAAAATCA1441080.3604297695060778No Hit
TAGAGATGTATTTATTAGATAAAAAATC1421940.35564264749456814No Hit
AGAGATGTATTTATTAGATAAAAAA1332140.3331826915576002No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC1322330.33072910394355054No Hit
TTACGCGAAACTCAGGTGCTGCAATCT1306630.3268023633175996No Hit
TGATGTATTTATTAGATAAAAAATCAAT1225450.30649836306188627No Hit
AGAGATGTATTTATTAGATAAAA1173850.293592642278506No Hit
TGATGTATTTATTAGATAAAAAATCAA1133030.2833831166510335No Hit
TCCAACGGGGCCTTTCCTTCTGGCT1024820.2563186196361192No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT1014050.25362492558889044No Hit
TTTGGAAGAGATGTATTTATTAGATAAA962900.24083175469606288No Hit
ACGTTCTAGCATTCAAGGTCCC949780.23755030010928097No Hit
TGCTTGGTGGGGCTTGCTCTGCTAGGCG926360.23169270358318084No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA856760.2142849871776912No Hit
ATATAGATGGATACGAATAAGGCGTCC768100.19211015763012348No Hit
TTGATGTATTTATTAGATAAAAAATCA766650.1917474968716758No Hit
GAGATGTATTTATTAGATAAAAAATCA752380.18817841478681463No Hit
TTGGAAGAGATGTATTTATTAGATAAA696500.1742022194888439No Hit
AACTATGCCGACTAGGGATCGGGTGGTG682820.17078070281604077No Hit
AGAGATGTATTTATTAGATAAAAAAT676760.16926503095659728No Hit
CAAGAGGTGCACAATCGACCGATCCTGA650980.16281717278226504No Hit
ACACGGAGTCGACCCGCAACGCGA641660.16048613949348398No Hit
TCTATGCCGACTAGGGATCGGGTGGTG637510.15944817939171987No Hit
TGGAAGAGATGTATTTATTAGATA606400.15166723029150747No Hit
CCAGATCGGGGTTCAATTCCCCGTCGCGGA593070.1483332524224676No Hit
ACGGAGTCGACCCGCAACGCGA590480.14768546527461962No Hit
TCGCGAAACTCAGGTGCTGCAATCT564620.14121759823085578No Hit
AGATGTATTTATTAGATAAAAAATCAATG562560.14070236984299217No Hit
TGGAAGAGATGTATTTATTAGATAA531630.13296644069900085No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT530740.13274384202657433No Hit
AAAAATAATATTTAAAATCAAATATAT497540.12444016121246144No Hit
CGATGTATTTATTAGATAAAAAATCA495570.12394744280270838No Hit
TCGTATGGCGCAGTGGTAGCGCAGCAGA488810.12225669333573841No Hit
ATGTATTTATTAGATAAAAAATCAATGT483660.12096862236607932No Hit
AGATGTATTTATTAGATAAAAAATC481470.12042087956538935No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCT480160.12009323432844694No Hit
ATGCCGACTAGGGATCGGGTGGTG459480.11492094158037905No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG458240.11461080410418928No Hit
TTTACCCAATCCTAATTCAGGGAGGTAG454280.11362036506732522No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA449250.11236230740181354No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGC445860.1115144315596496No Hit
GATGTATTTATTAGATAAAAAATCA444570.1111917885400651No Hit
TTCCAACGGGGCCTTTCCTTCTGGC438740.10973364218023744No Hit
AGAGATGTATTTATTAGATAAAAAATCAA428200.10709747363262449No Hit
TTGATGTATTTATTAGATAAAAAATCAATG410260.1026104846625888No Hit
TAGAGATGTATTTATTAGATAAAAA406220.10160003675629314No Hit
GATGTATTTATTAGATAAAAAATCAATG402090.10056707887188693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)