FastQCFastQC Report
Sun 1 Oct 2023
SRR7471272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8730478
Total Bases247.1 Mbp
Sequences flagged as poor quality0
Sequence length20-45
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA210692024.13292834596227No Hit
ACGCGAAACTCAGGTGCTGCAATCT1765642.022386403127068No Hit
ATGTATTTATTAGATAAAAAATCAATG1428851.6366228744863685No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1141651.307660359490053No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC978471.12075192217425No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG785620.8998590913349761No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAG665600.7623866642811539No Hit
AGAGATGTATTTATTAGATAAAAAATCA622830.7133973649552751No Hit
TCCGATATAGTGTAACGGCTATCACATCACG544160.6232877512548568No Hit
ATGTATTTATTAGATAAAAAATCA541330.6200462334364739No Hit
ACGTTCTAGCATTCAAGGTCCCA489590.5607825825802436No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC481450.5514589235549302No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCG477360.54677418578914No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGC415060.4754149772784491No Hit
ACACGGAGTCGACCCGCAACGCGA403120.4617387501577806No Hit
TACGCGAAACTCAGGTGCTGCAATCT401580.4599748146665051No Hit
ACGGAGTCGACCCGCAACGCGA395230.4527014442966353No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT355320.40698802516883953No Hit
TGATGTATTTATTAGATAAAAAATCAATG347070.3975383707512922No Hit
ATGTACACGGAGTCGACCCGCAACGC346990.39744673773875844No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG335800.3846295701105942No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG323860.37095334298992566No Hit
GTCTAGTGGTATGATTCTCGCT304280.3485261631722799No Hit
TGATGTATTTATTAGATAAAAAATCA265470.3040726979668238No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT233930.2679463827753761No Hit
ATAGTGTAACGGCTATCACATCACGC220390.2524374954040317No Hit
AGAGATGTATTTATTAGATAAAAAATC214980.24624081293143396No Hit
ATTTATTAGATAAAAAATCAATG208490.2388070847896301No Hit
TACACGGAGTCGACCCGCAACGCGA206440.23645898884345162No Hit
GTCTAGTCGGTTATGGCATCTGCT202640.2321064207480965No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGC197390.22609300430056636No Hit
ATGTACACGGAGTCGACCCGCAACGCG193280.22138535828164274No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA177980.2038605446345549No Hit
ACGTTCTAGCATTCAAGGTCCC177250.2030243933951841No Hit
ATATAGTGTAACGGCTATCACATCACGC175540.20106573775227427No Hit
ATGTATTTATTAGATAAAAAATCAAT164000.1878476756942747No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA158240.18125009879184165No Hit
AAGAGGTGCACAATCGACCGATCCTGA153720.17607283358368236No Hit
TTGTCCGCGCGAGTTCGAACCTCGCATCCT153100.17536267773654546No Hit
TTGTCCGCGCGAGTTCGAACCTCGCATCCTT143660.16454998225755793No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT141190.16172081299557708No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG140520.1609533865156066No Hit
ATAGTGTAGTGGTTATCACTTTCGGT134890.15450471325854093No Hit
TGTACACGGAGTCGACCCGCAACGCGA132770.15207643842639543No Hit
ATCGAAGGGTTGCAGGTTCATTTCCTGTCCGT126070.1444021736266903No Hit
AAGCGGTGGCTCAATGGTAGAGCTTTCGACT122820.14067958249250498No Hit
ATTTATTAGATAAAAAATCA120490.1380107710024583No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA118930.13622392725804933No Hit
AGATGCAAGAGTTCGAATCTCTTAGCAACCA115190.13194008392209453No Hit
GTTTAGTGGTAAAATCCAACGT113560.1300730612917185No Hit
AAAACCCTGGTTCGATTCTAGGAGATGGC111290.12747297456107215No Hit
ACCTGCGCAGGTTCGAATCCTGTCTGTGACGC107290.12289132393438253No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCA105220.12052031973507063No Hit
AGCGTGTTACCTATACTCTACCGTCAGGGT104600.11981016388793375No Hit
GCGTAGTCGGTAGCGCTCTCCCT102960.117931687130991No Hit
ACCTGCGCAGGTTCGTATCCTGTCTGTGACGC99070.11347603189653534No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC98870.11324694936520084No Hit
ATACGCGAAACTCAGGTGCTGCAATCT98500.11282314668223205No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA98350.11265133478373118No Hit
TAGTGTAACGGCTATCACATCACGC97980.11222753210076242No Hit
AAACCCTGGTTCGATTCTAGGAGATGGC96950.11104775706438984No Hit
GCTCAATGGTAGAGCTTTCGACT96650.11070413326738811No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGA95790.10971907838264984No Hit
ATGTATTTATTAGATAAAAAATCAA95230.10907764729491329No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC94340.10805823003047486No Hit
TATACGCGAAACTCAGGTGCTGCAATCT94270.10797805114450777No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCC93790.10742825306930502No Hit
ATCAAACAGTTATCCCTGTCTGAATGGGTA92990.10651192294396711No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGT91240.10450745079479037No Hit
ATATAGTGTAACGGCTATCACATCACG89150.10211353834234506No Hit
ATAGTGTAACGGCTATCACATCACG88970.10190736406414402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)