FastQCFastQC Report
Sun 1 Oct 2023
SRR7471273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48809471
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length20-45
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTTATTAGATAAAAAATCAATG24317214.982067926939835No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA19014673.8956927027543484No Hit
ACGTTCTAGCATTCAAGGTCCCA17190153.521888200755136No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT16691413.4197072121515104No Hit
AAGAGGTGCACAATCGACCGATCCTGA9743301.9961904524636211No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA9325391.9105697744603707No Hit
TGATGTATTTATTAGATAAAAAATCAATG8702761.7830064169308453No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC7639911.5652515471843569No Hit
ACGTTCTAGCATTCAAGGTCCC5919201.2127154584404327No Hit
ACGCGAAACTCAGGTGCTGCAATCT5757151.1795149347142073No Hit
ATGTATTTATTAGATAAAAAATCA5316821.089300885887495No Hit
AGAGATGTATTTATTAGATAAAAAATCA4336100.8883726684929653No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA4117470.843580132224748No Hit
ATGTACACGGAGTCGACCCGCAACGCGA3739310.7661033654718364No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA3039680.6227643811177548No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG2531950.5187415368627945No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT2522450.5167951932935311No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA2134730.4373597902751292No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC2099130.43006612384715254No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA2084870.4271445597105529No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC2051110.42022786930020195No Hit
ATTTATTAGATAAAAAATCAATG2042210.4184044526932078No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1925350.3944623780085631No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT1886270.3864557351994247No Hit
TGATGTATTTATTAGATAAAAAATCA1734840.35543101870536564No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC1695450.34736086363238805No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1555910.31877214977396495No Hit
ACTATGCCGACTAGGGATCGGGTGGTG1509810.3093272615062761No Hit
TCCAACGGGGCCTTTCCTTCTGGC1325590.2715845865242014No Hit
ACGTTCTAGCATTCAAGGTCC1234410.2529037858246814No Hit
AAGAGGTGCACAATCGACCGATCCTGAT1172150.24014806470654027No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCT1139720.23350386239588622No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1138160.23318425229398612No Hit
ATGTATTTATTAGATAAAAAATCAA1105340.2264601474578571No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCA1095210.22438473057821096No Hit
ATGTATTTATTAGATAAAAAATCAAT1083850.22205731342591276No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGG1007840.2064845160891008No Hit
TCCAACGGGGCCTTTCCTTCTGGCT999140.2047020751361964No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGT926200.18975825408966224No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCT916630.18779756904146738No Hit
AACGGGGCCTTTCCTTCTGGCTAACCT914630.18738781250056982No Hit
TCCGATATAGTGTAACGGCTATCACATCACG910440.1865293725473894No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTT886430.18161024527391414No Hit
AGAGATGTATTTATTAGATAAAAAATCAA772350.15823773218111706No Hit
AGAGATGTATTTATTAGATAAAAAATC738700.15134357838051554No Hit
TCCACGTTCTAGCATTCAAGGTCCCA731430.1498541133543529No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG716370.14676864660139421No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC706680.14478337616074552No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT704930.14442483918746016No Hit
TACGCGAAACTCAGGTGCTGCAATCT699090.14322835008803927No Hit
ATCAGCTTGCGTTGATTACGTCCCTGCCCT678790.139069321197929No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT661490.13552492711916506No Hit
AGATGTATTTATTAGATAAAAAATCAATG660050.1352299024097188No Hit
ACGGGGCCTTTCCTTCTGGCTAACCT637750.13066111697871097No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG635240.1301468725198845No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATT630950.12926794473965925No Hit
TCACGTTCTAGCATTCAAGGTCCCA630660.1292085300412291No Hit
ATTTCCACGTTCTAGCATTCAAGGTCCCA628210.12870657827862955No Hit
TTGATGTATTTATTAGATAAAAAATCAATG613830.12576042874957608No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTT600640.12305808436235664No Hit
TTTTCCACGTTCTAGCATTCAAGGTC598020.12252130329378083No Hit
AGCGGAGAATTTGGACAAACTTGGTCATT581830.11920432409521504No Hit
TAGAGATGTATTTATTAGATAAAAAATCA581780.11919408018169261No Hit
ATTACCCAATCCTAATTCAGGGAGGTAG576760.11816559126403972No Hit
ATGATTAATAGGGACGGTCGGGGGC524180.10739309180384275No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTT520100.10655718846041172No Hit
GTCTAGTCGGTTATGGCATCTGCT519280.1063891882786437No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGA510950.10468255228580535No Hit
ATCAGCTTGCGTTGATTACGTCCCTGCCC505190.10350245344802037No Hit
ATGTATTTATTAGATAAAAAATCAATGT504190.10329757517757159No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA491850.10076937732023362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)