FastQCFastQC Report
Sun 1 Oct 2023
SRR7471274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46027416
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length20-45
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA461424210.024985977922375No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA15631123.396045522086228No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC9509722.066099039754915No Hit
TCCGATATAGTGTAACGGCTATCACATCACG6843991.4869376981753657No Hit
ACGTTCTAGCATTCAAGGTCCCA5551291.206083348237494No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG5413121.1760642830785895No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA4656421.0116622666803627No Hit
GTCTAGTCGGTTATGGCATCTGCT4373250.9501402381571887No Hit
ACGCGAAACTCAGGTGCTGCAATCT4167470.9054321015978825No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAG4132060.8977388606825115No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCG4060940.882287200306878No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT3328370.7231277115360984No Hit
AGAGATGTATTTATTAGATAAAAAATCA2492960.5416250175764808No Hit
TACGCGAAACTCAGGTGCTGCAATCT2399390.5212958294247932No Hit
ATGTATTTATTAGATAAAAAATCAATG2308230.5014902422504014No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA1889940.410611797108054No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTG1673940.3636832447861075No Hit
ACGTTCTAGCATTCAAGGTCCC1643950.3571675629151113No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC1557530.3383917967500065No Hit
ATCGAAGGGTTGCAGGTTCATTTCCTGTCCGT1527980.3319717100781847No Hit
AAAACCCTGGTTCGATTCTAGGAGATGGC1515800.32932546115558603No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA1504570.32688561095847746No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1495650.3249476355570341No Hit
AAGAGGTGCACAATCGACCGATCCTGA1460790.31737388864063104No Hit
ACGGAGTCGACCCGCAACGCGA1327410.2883955075818291No Hit
ATCGAAGGGTTGCAGGTTCATTTCCTGTCCG1246200.270751675479675No Hit
GTCTAGTCGGTTATGGCATCTGC1231070.26746450419897566No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG1210330.26295849412880357No Hit
CTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1174690.2552152829956824No Hit
ACACGGAGTCGACCCGCAACGCGA1130530.24562100118764No Hit
ATGTATTTATTAGATAAAAAATCA1128590.2451995132640077No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA1105830.24025463432489888No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGT1073590.23325011336721574No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC1052470.22866154380684767No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTC1045960.2272471693827001No Hit
TCCAACGGGGCCTTTCCTTCTGGC1035180.22490508700292886No Hit
ATCGGGCGTTCGACTCGCCCCCGGGAGA993890.2159343466076827No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC967450.21018994418457038No Hit
GTTTAGTGGTAAAATCCAACGT934980.20313545300913702No Hit
AAACCCTGGTTCGATTCTAGGAGATGGC929500.2019448582557839No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT906840.1970217054983056No Hit
TTGTCCGCGCGAGTTCGAACCTCGCATCCT899680.1954661108935596No Hit
GTCTAGTGGTATGATTCTCGCT862560.18740135227230656No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA830110.18035120633319932No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGC828960.18010135524444823No Hit
AGGTGGGGACACGGGTTCGATTCTCGTACCGC783470.17021811522071975No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG775160.16841266952722264No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG760970.16532972435385032No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGCT743230.16147549973259415No Hit
TGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCC741600.1611213629720165No Hit
TCCGTGATAGTTTAATGGTCAGAATGGG738440.16043481563249173No Hit
AGAGATGTATTTATTAGATAAAAAATC711290.15453615731980264No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGT708060.15383440165313647No Hit
ACCTGCGCAGGTTCGAATCCTGTCTGTGACG706760.15355196129193957No Hit
TGATGTATTTATTAGATAAAAAATCAATG695250.15105127778626548No Hit
ACCTGCGCAGGTTCGTATCCTGTCTGTGACG689340.1497672604519011No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG651190.1414787221598536No Hit
AAAACCCTGGTTCGATTCTAGGAGATGG647300.14063357369442595No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT644050.1399274727914337No Hit
TGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCC639650.13897152079969036No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG620200.1347457784725521No Hit
AGATCGGGGTTCAATTCCCCGTCGCGGA615720.13377244553550433No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG608220.1321429819132145No Hit
GTCTAGTCGGTTATGGCATCTGCTT606010.13166283329917977No Hit
GTCTAGTGGTATGATTCTCGC599700.13029191123829328No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCC588430.12784337056853246No Hit
ATGCCGACTAGGGATCGGGTGGTGTT586410.12740450169959572No Hit
ATCTGGTTGATCCTGCCAGTAGTC568580.1235307235148721No Hit
AGGAAGAGGTCATCGGTTCGATTCCGGTTG565500.12286155712065173No Hit
TACACGGAGTCGACCCGCAACGCGA564580.12266167624965087No Hit
GCCCAGTTGGTTAAGGCACCGT561300.12194905749216944No Hit
GTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA557640.12115387924449203No Hit
TAACCCTGGTTCGATTCTAGGAGATGGC552840.12011102252622655No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG546340.11869882072024203No Hit
ATCGAAGGGTTGCAGGTTCATTTCCTGTC543440.11806876145295665No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC521100.11321513247669605No Hit
AGGTTAGGGGTTCGAGCCCCCTACAGGGCT520020.11298048971508633No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG516270.11216575790394141No Hit
TCGAAGGGTTGCAGGTTCATTTCCTGTCCGT507650.11029296104738966No Hit
TCCGATATAGTGTAACGGCTATCACATC507450.11024950868412861No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGC505880.10990840763252928No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCC503130.10931093763768968No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTT498770.10836367611859854No Hit
AGATCGGGGTTCAATTCCCCGTCGCGGAG494380.10740989674501823No Hit
ATGCCAGATCGGGGTTCAATTCCCCGTCGCGGA491650.10681677198650474No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC483450.10503522509280122No Hit
TCCGATATAGTGTAACGGCTATCACATCAC482330.10479189185853928No Hit
GCCTTGTTGGCGCAATCGGTAGCGCTTATGA479220.10411620760982976No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG479210.10411403499166672No Hit
TTGCCCGCGCTGGTTCAAATCCTGCTGGTG472930.10274963078526937No Hit
GCTCAATGGTAGAGCTTTCGACT471730.102488916605703No Hit
ATCGAAGGGTTGCAGGTTCATTTCCTGTCC470370.10219344053552777No Hit
TAAACCCTGGTTCGATTCTAGGAGATGGC467910.10165897646741673No Hit
TGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCC466360.1013222206521435No Hit
AAGGTTAGGGGTTCGAGCCCCCTACAGGGCT464230.10085945298341319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)