FastQCFastQC Report
Sun 1 Oct 2023
SRR7471275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47138695
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length20-45
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTTATTAGATAAAAAATCAATG34499057.318626449035129No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA19726944.184871897705271No Hit
ACGTTCTAGCATTCAAGGTCCCA18016323.8219810709651596No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC17711693.7573568805839876No Hit
AAGAGGTGCACAATCGACCGATCCTGA14223103.0172876020432895No Hit
ACGTTCTAGCATTCAAGGTCCC11209622.378008131111818No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT7630351.6187020026752967No Hit
TGATGTATTTATTAGATAAAAAATCAATG5333911.1315353554017564No Hit
ACGCGAAACTCAGGTGCTGCAATCT4664070.9894355369829393No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA4552900.9658519396856446No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC3207040.6803412780094994No Hit
ATGTACACGGAGTCGACCCGCAACGCGA3202420.6793611914797387No Hit
ATTTATTAGATAAAAAATCAATG2390190.5070547667897891No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC2369590.5026846839947521No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC2253800.4781210001676966No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT2017890.4280750665668619No Hit
ATGCCGACTAGGGATCGGGTGGTGTT1910920.4053824570238951No Hit
TCGGGGCTGATCCGGGTTGAAGAC1765790.37459458731303447No Hit
ACTATGCCGACTAGGGATCGGGTGGTG1489880.3160630560519336No Hit
AGAGATGTATTTATTAGATAAAAAATCA1482200.31443382130116243No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG1481950.3143807863157858No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1341320.28454754634170504No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1339010.28405750307682465No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA1313570.2786606629648954No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT1295130.2747488024435127No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTC1196060.2537320984384485No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCT1137190.2412434200819518No Hit
ACGTTCTAGCATTCAAGGTCC1082290.2295969372932365No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC1065610.22605844306890552No Hit
AACGGGGCCTTTCCTTCTGGCTAACCT1046790.22206596936975026No Hit
TCCAACGGGGCCTTTCCTTCTGGC1025710.21759405940279No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA996560.21141018010787105No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCT991410.2103176594091118No Hit
ATGTATTTATTAGATAAAAAATCAAT920840.1953469437369872No Hit
ATGTATTTATTAGATAAAAAATCA902590.19147538980449078No Hit
ATGCCGACTAGGGATCGGGTGGTGTTT895880.19005193079698113No Hit
AAGAGGTGCACAATCGACCGATCCTGAT892440.18932216939819824No Hit
ACAGCCAAGGGAACGGGCTTGGCAGAATC881300.18695893044981413No Hit
AAAAGTCTTTGGGTTCTGGGGGGAGT881220.18694195925449358No Hit
ACGTGAAATTGTTGAAAGGGAAGGGC877720.18619946945922028No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT826870.17541215343360694No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA811990.17225551110398793No Hit
AAGGCGTCCTTGTGGCGTCGCTGAACCATAGC798090.16930676591704544No Hit
TTCCACGTTCTAGCATTCAAGGTCCCA745880.15823093957098305No Hit
ATGAAAGTGTGGCCTATCGATCCTTT738090.15657836942664619No Hit
TCCACGTTCTAGCATTCAAGGTCCCA730490.15496610587119564No Hit
AGAGATGTATTTATTAGATAAAAAATC700800.14866767100786307No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG661950.14042603428032957No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT642980.13640173958994833No Hit
AAGTGGGTGGTAAATTCCATCTAAAGCT637140.1351628423315495No Hit
ATGCGAGTGTTTGGGTGTAAAACCCAT633250.1343376179590886No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTT628140.1332535828579896No Hit
TCCAACGGGGCCTTTCCTTCTGGCT622070.1319658934130442No Hit
TACGCGAAACTCAGGTGCTGCAATCT616900.1308691299154548No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC601780.1276615739998742No Hit
ATCGATGAAGAACGCAGCGAAATGCGAT596330.12650541131866294No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGC546570.11594932782929183No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACC545870.11580082987023717No Hit
TTGTGGCGTCGCTGAACCATAGC527030.11180411337225182No Hit
ACCAGGACTTTTACTTTGAAAAAATT517920.10987151850512619No Hit
TCACGTTCTAGCATTCAAGGTCCCA508490.10787103885671846No Hit
AAGAGGTGCACAATCGACCGATCCTG506220.10738948118949834No Hit
ACAATGTAAATACCTTAACGAGGAAC483150.10249541273893985No Hit
TCCACGTTCTAGCATTCAAGGTCCC481950.10224084480913187No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTT479750.10177413693781723No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCA477760.10135197845421898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)