FastQCFastQC Report
Sun 1 Oct 2023
SRR7471277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50147621
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length20-45
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA620264012.368762219049234No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC43898068.753767202635593No Hit
ATGTATTTATTAGATAAAAAATCAATG41305948.236869302334402No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA20291474.0463474827649355No Hit
ACGTTCTAGCATTCAAGGTCCC19789193.9461871979929017No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC18529663.695022740959138No Hit
ATGTACACGGAGTCGACCCGCAACGCGA13021692.596671535026557No Hit
ACGTTCTAGCATTCAAGGTCCCA11253082.2439907966920307No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC9860571.966308631071452No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA9646401.9236007227541263No Hit
CAAGAGGTGCACAATCGACCGATCCTGA4293670.8562061199274039No Hit
TTTCCACGTTCTAGCATTCAAGGTCC3876290.7729758506390562No Hit
ACGTTCTAGCATTCAAGGTCC3731180.7440392835384952No Hit
ACGCGAAACTCAGGTGCTGCAATCT3394300.6768616202152441No Hit
TTCCACGTTCTAGCATTCAAGGTCCC3309610.6599734810949456No Hit
AAGAGGTGCACAATCGACCGATCCTG2955790.5894177911251264No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA2667120.5318537443680529No Hit
TTTTCCACGTTCTAGCATTCAAGGTC2595960.517663639517416No Hit
TCCACGTTCTAGCATTCAAGGTCCC2590160.5165070542429121No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT2586630.5158031325154986No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA2507550.5000336905314013No Hit
AGAGGTGCACAATCGACCGATCCTGA2082850.41534373086212806No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCT1970720.39298374692590105No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT1920030.38287559044924585No Hit
AAGAGGTGCACAATCGACCGATCCTGAT1898660.37861417194646185No Hit
TGATGTATTTATTAGATAAAAAATCAATG1884830.37585631430053285No Hit
ATGTATTTATTAGATAAAAAATCA1850470.36900454360536866No Hit
TTCCACGTTCTAGCATTCAAGGTCCCA1812560.36144486295770645No Hit
TCCACGTTCTAGCATTCAAGGTCCCA1705880.3401716703570046No Hit
ATGTATTTATTAGATAAAAAATCAAT1384060.27599714052237895No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1279830.25521250549452784No Hit
AAAGAGGTGCACAATCGACCGATCCTGA1253380.24993807782028185No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCT1069860.21334212444494624No Hit
TTTCCACGTTCTAGCATTCAAGGTC984610.19634231502228192No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC978640.19515182983455984No Hit
ATTTCCACGTTCTAGCATTCAAGGTCCC960700.1915743919337669No Hit
ATGATTAATAGGGACGGTCGGGGGC911200.18170353484963925No Hit
ACTATGCCGACTAGGGATCGGGTGGTG872840.17405411913757582No Hit
ATATAGATGGATACGAATAAGGCGTCC831440.16579849321266904No Hit
ACGTTCTAGCATTCAAGGTC794900.1585120059832948No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC744910.14854343738459697No Hit
ATGTATTTATTAGATAAAAAATCAA708640.14131079119386342No Hit
AACGGGGCCTTTCCTTCTGGCTAACCT672360.1340761508905876No Hit
TAAGAGGTGCACAATCGACCGATCCTGA640710.12776478469437264No Hit
TTCCACGTTCTAGCATTCAAGGTCC621230.1238802534620735No Hit
ACGGGGCCTTTCCTTCTGGCTAACCT599380.11952311755726158No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTT565420.11275111136378733No Hit
AAGAGGTGCACAATCGACCGATCCT563640.11239615933126718No Hit
TCACGTTCTAGCATTCAAGGTCCCA510010.10170173376719106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)