FastQCFastQC Report
Sun 1 Oct 2023
SRR7471278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27989844
Total Bases749.5 Mbp
Sequences flagged as poor quality0
Sequence length20-45
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA419769214.997196840396823No Hit
ACGCGAAACTCAGGTGCTGCAATCT5119121.828920518456623No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC4683771.6733819595421824No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC4497241.6067399303833205No Hit
ACGTTCTAGCATTCAAGGTCCC3852931.3765457213695083No Hit
ACGTTCTAGCATTCAAGGTCCCA3561541.2724401036318744No Hit
AAGAGGTGCACAATCGACCGATCCTGA3297411.1780737327439195No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA2999641.071688716807425No Hit
GTTTAGTGGTAAAATCCAACGT2270410.8111549317673938No Hit
GTCTAGTCGGTTATGGCATCTGC2251740.8044846552199434No Hit
ATGTATTTATTAGATAAAAAATCAATG2185850.7809439738213617No Hit
TCCGATATAGTGTAACGGCTATCACATCACG2099550.7501113618210948No Hit
GTCTAGTCGGTTATGGCATCTGCT2006340.7168099972261368No Hit
GTCTAGTCGGTTATGGCATCTG1880660.671907996343245No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC1566590.559699439553861No Hit
TCCAACGGGGCCTTTCCTTCTGGC1522790.5440509064645019No Hit
ACACGGAGTCGACCCGCAACGCGA1446580.5168231734339069No Hit
ACGGAGTCGACCCGCAACGCGA1406250.5024143757285678No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1276700.4561297304836711No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGG1188870.42475049164261147No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGC1137660.40645456973607996No Hit
ACGTTCTAGCATTCAAGGTCC1088800.3889982380752104No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG1073450.38351410604503544No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGT993650.355003764937025No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA977740.34931956033767103No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC957320.34202405701153604No Hit
TACACGGAGTCGACCCGCAACGCGA953950.3408200488720123No Hit
ATGTATTTATTAGATAAAAAATCA952360.34025198568452186No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT896280.32021614697102274No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA863320.30844044718505753No Hit
GTTTAGTGGTAAAATCCAACG826270.2952035030991956No Hit
ATCTGGTTGATCCTGCCAGTAGTC818670.29248823251748024No Hit
TCGGGGCTGATCCGGGTTGAAGAC818610.29246679617078253No Hit
TACGCGAAACTCAGGTGCTGCAATCT783440.2799015242814501No Hit
GTCTAGTGGTATGATTCTCGC737310.26342054639532825No Hit
TTGGGTACAAATGGCAGTCTGACAAGT730290.26091249383169124No Hit
ATGTACACGGAGTCGACCCGCAACGC729990.26080531209820246No Hit
AAGAGGTGCACAATCGACCGATCCTG685940.2450674608976027No Hit
ATGCCGACTAGGGATCGGGTGGTGTT668950.2389974020576892No Hit
TATCTGGTTGATCCTGCCAGTAGTC607720.21712161025263305No Hit
TTGGGGACACGGGTTCGATTCTCGTACCG600930.21469573035133743No Hit
TCGTATGGCGCAGTGGTAGCGCAGCAGA598890.21396689456361387No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC593630.2120876415031109No Hit
GCCCAGTTGGTTAAGGCACCG572650.20459206560779689No Hit
GTCTAGTGGTATGATTCTCGCT563460.20130873183859116No Hit
GCCCAGTTGGTTAAGGCACCGT559460.199879642058741No Hit
TGGGGACACGGGTTCGATTCTCGTACCGC535760.19141228511312888No Hit
TCCGTGATAGTTTAATGGTCAGAATGGG519640.1856530533003328No Hit
TCCAACGGGGCCTTTCCTTCTGGCT489310.17481698004461904No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG475910.17002952928212106No Hit
TCGTGGTCTAGTCGGTTATGGCATCTGC473760.16926139352545158No Hit
TCTACATGGTATAACTGTGGTAATTCT464230.1658565871249586No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT445330.15910413791516664No Hit
TCGTGGTCTAGTCGGTTATGGCATCTG445230.1590684106706704No Hit
TCCGATATAGTGTAACGGCTATCACATC433680.1549419139313531No Hit
AAGCCTTTGTTGTCTGATTTGT419530.1498865088351332No Hit
AAATTCTAGAGCTAATACATGCTT393070.14043307994142448No Hit
CCAGATCGGGGTTCAATTCCCCGTCGCGGA389970.13932553536204062No Hit
ACGTGAAATTGTTGAAAGGGAAGGGC386490.13808222725357097No Hit
ATGTACACGGAGTCGACCCGCAACGCG382040.13649236487348768No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTG381830.13641733766004555No Hit
ACAGCCAAGGGAACGGGCTTGGCAGAATC367490.13129405079928277No Hit
ATTTATTAGATAAAAAATCAATG367480.13129047807483313No Hit
CAAGAGGTGCACAATCGACCGATCCTGA366150.13081530572303296No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT357350.12767130820736264No Hit
TCCGTGATAGTTTAATGGTCAGAATG354820.12676740892160743No Hit
ACTATGCCGACTAGGGATCGGGTGGTG353020.12612431852067485No Hit
ATAGTGTAGTGGTTATCACTTTCGGT330870.11821073386475466No Hit
TATACAGTGAAACTGCGAATGGCTC330320.11801423402002527No Hit
AAGGCGTCCTTGTGGCGTCGCTGAACC320080.11435576418360888No Hit
GCGTAGTCGGTAGCGCTCTCCCT318720.11386987365845982No Hit
AGATCGGGGTTCAATTCCCCGTCGCGGA318170.11367337381373044No Hit
ATGTATTTATTAGATAAAAAATC315750.11280877449692109No Hit
TCGTGGTCTAGTCGGTTATGGCATCTGCT313630.1120513569136005No Hit
AGTTTAATGGTCAGAATGGGC311670.11135110292147396No Hit
TCCACGTTCTAGCATTCAAGGTCCCA309740.11066156710269626No Hit
GTCTAGTGGTATGATTCTCG302760.10816780543685774No Hit
TGATGTATTTATTAGATAAAAAATCAATG300080.10721031528435813No Hit
ATGCCGACTAGGGATCGGGTGGTGTTT296890.10607061618492764No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCT294820.10533106222385519No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA289950.10359114541688763No Hit
TTGTACACGGAGTCGACCCGCAACGCGA285520.1020084284857036No Hit
AAATAGAATAGGACGTTTGGTTCTAT284060.10148681071605828No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG280240.1001220299763014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)