FastQCFastQC Report
Sun 1 Oct 2023
SRR7471279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6710861
Total Bases342.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1635742.4374517666213023Illumina Single End Adapter 1 (95% over 22bp)
ACGNTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG1500832.2364194400688673No Hit
AAGNGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1099311.6381057512590411No Hit
AGANATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA676631.0082610860216No Hit
ATGNACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA620250.9242480212300628No Hit
ACTNTGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA329270.490652391697578No Hit
TCANGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT264870.3946885503961414No Hit
AAGNTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG250770.37367783358946044No Hit
ATGNATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT245830.3663166320983254No Hit
ACANGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT186350.27768418985283705No Hit
AGANGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT183560.2735267501442811No Hit
ACGNTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT182950.2726177758710842No Hit
ATTNATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG169530.25262034186075377No Hit
TGANGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT167010.24886523502721933No Hit
CAANAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA155120.23114768730867769No Hit
AGANATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT152020.22652830985472652No Hit
ACGNGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT146930.21894359009969064No Hit
TCCNACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG137010.20416158224704697No Hit
AGANATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC130160.1939542481955743No Hit
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC129920.1935966189733329Illumina Single End Adapter 1 (95% over 22bp)
CCANCGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT128450.19140613998710448No Hit
ACGNGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC124800.18596719556551686No Hit
ATGNTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG100050.1490866820218747No Hit
AAGNGGTGCACAATCGACCGATCCTGATCTGTAGGCACCATCAATTCGTA95940.14296228159099109No Hit
TTTNCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT90710.13516894478964772No Hit
AAGNGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT89520.1333956998960342No Hit
TTGNCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT84630.1261090044928661No Hit
TAANGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT75230.11210185995507878No Hit
TAGNGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT72020.10731856910760035No Hit
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT71310.1062605826584696Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)