FastQCFastQC Report
Sun 1 Oct 2023
SRR7471280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94022976
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length20-45
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA21152532.2497192601093587No Hit
ATGTACACGGAGTCGACCCGCAACGCGA16560381.7613120435583745No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCA15545301.6533511979029465No Hit
ACGTTCTAGCATTCAAGGTCCCA12895651.3715424195890162No Hit
AAGAGGTGCACAATCGACCGATCCTGA12650041.3454200811512285No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA10813991.1501433436865474No Hit
TCCGATATAGTGTAACGGCTATCACATCACG10725671.140749895004387No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA9196770.9781407046720154No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC8503150.9043693745664889No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC6848120.7283453780488719No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAG6396440.6803060562558667No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCG5871330.6244569412480626No Hit
ACGTTCTAGCATTCAAGGTCCC5732510.6096924649566505No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG5046750.5367571007324848No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC4592480.48844231435516355No Hit
GCGTAGTCGGTAGCGCTCTCCCT4354700.4631527510892657No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT3791380.4032397357854318No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA3742420.39803249792901685No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG3581820.38095156656177315No Hit
GTCTAGTCGGTTATGGCATCTGCT3373120.3587548643429453No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT3190010.3392798373027461No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG3162940.3364007537902225No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA3127870.3326708144188076No Hit
ACGCGAAACTCAGGTGCTGCAATCT2847710.3028738422404328No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG2771580.29477688517325806No Hit
TACGCGAAACTCAGGTGCTGCAATCT2745210.29197225154838746No Hit
AGAGATGTATTTATTAGATAAAAAATCA2737740.29117776489014774No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC2681680.2852153924589666No Hit
ATCGAAGGGTTGCAGGTTCATTTCCTGTCCGT2609380.27752578263423616No Hit
TAGCGTGTTACCTATACTCTACCGTCAGGGT2563840.2726822856574972No Hit
TGATGTTGACGCAATGTGATTTCTGCCCA2536760.26980213857515No Hit
GTTTAGTGGTAAAATCCAACGT2492970.2651447663175435No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGA2188200.23273034880325422No Hit
AGCGTGTTACCTATACTCTACCGTCAGGGT2131310.22667970007671318No Hit
GTCTAGTGGTATGATTCTCGCT2053990.21845617820052837No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC2040080.216976752575881No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC1946020.20697281481496607No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT1890560.20107425657320183No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1882170.20018192149118957No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGA1809770.1924816759682229No Hit
TTGATGTTGACGCAATGTGATTTCTGCC1793880.1907916635185No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG1779630.1892760765198498No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCC1778450.1891505752806633No Hit
ATGTATTTATTAGATAAAAAATCAATG1768620.18810508614405058No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG1754320.1865841812962823No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGC1684250.17913174754221778No Hit
CTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1663350.17690888661086415No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG1612460.17149637977849158No Hit
GTCTAGTCGGTTATGGCATCTGC1562130.16614343285624142No Hit
ATCCGTGATAGTTTAATGGTCAGAATGGGC1555060.16539148899094622No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTC1448770.15408680533575111No Hit
AAAACCCTGGTTCGATTCTAGGAGATGGC1440170.15317213528744295No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG1399240.1488189439993901No Hit
GCGTAGTCGGTAGCGCCTCCCT1356450.14426792872414504No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG1347770.14334475011724795No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG1332980.14177173034812257No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGCT1324660.14088684025487558No Hit
ACCTGCGCAGGTTCGTATCCTGTCTGTGACG1314140.13976796480043346No Hit
CCAACGGGGCCTTTCCTTCTGGC1277510.13587210853653475No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA1247290.1326580005295727No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCC1246430.13256653352474188No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT1243030.13220491978471305No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC1230120.13083185114242712No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGC1217160.12945346465102317No Hit
GTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1207770.12845477258664945No Hit
AAGCGGTGGCTCAATGGTAGAGCTTTCGACT1192470.12682751075651977No Hit
AAGCGGTGGCTCAATGGTAGAGCTTTCGA1188740.12643079921231168No Hit
AGGTGGGGACACGGGTTCGATTCTCGTACCGC1181270.12563631255407187No Hit
TTACGCGAAACTCAGGTGCTGCAATCT1176190.1250960190836759No Hit
TTGCCCGCGCTGGTTCAAATCCTGCTGGTGTCG1125130.1196654315643019No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC1125040.11965585943588938No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGC1036190.11020604155307741No Hit
TATCCGTGATAGTTTAATGGTCAGAATGGGC1018760.1083522393505179No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT1007740.10718018540489507No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGC1006510.10704936631659052No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAG1001280.10649311929884031No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA990750.1053731802745746No Hit
TAGAGATGTATTTATTAGATAAAAAATCA974490.10364381574137795No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT973060.10349172525660111No Hit
TATACGCGAAACTCAGGTGCTGCAATCT971780.10335558831917849No Hit
TCACGTTCTAGCATTCAAGGTCCC969400.10310245870115832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)