FastQCFastQC Report
Wed 4 Oct 2023
SRR7471281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192893866
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length20-45
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA174060809.023656563552933No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC96538315.0047371646333225No Hit
ACGTTCTAGCATTCAAGGTCCC80009094.147829667118601No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA48835352.5317212523492065No Hit
ACGTTCTAGCATTCAAGGTCCCA44328122.2980575235088088No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA31572271.6367689991759509No Hit
ACGCGAAACTCAGGTGCTGCAATCT29872711.5486604431475286No Hit
ATGTATTTATTAGATAAAAAATCAATG22456281.1641780252359089No Hit
CAAGAGGTGCACAATCGACCGATCCTGA19723531.0225068535875579No Hit
CCAACGGGGCCTTTCCTTCTGGC16201840.8399354700060809No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC16011050.8300445385857941No Hit
ACTATGCCGACTAGGGATCGGGTGGTG14461210.7496977638469852No Hit
TCACGTTCTAGCATTCAAGGTCCC14413930.7472466750186862No Hit
ATGTATTTATTAGATAAAAAATCA13635410.7068866565202234No Hit
ACGTTCTAGCATTCAAGGTCC13249290.6868694310891151No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCT10949020.567618879078301No Hit
AAAGAGGTGCACAATCGACCGATCCTGA9003370.4667525301193352No Hit
TTGCCTTGTTGTAGACGGCCTTGG8930960.4629986523262487No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC7688220.3985725497357184No Hit
TCCAACGGGGCCTTTCCTTCTGGC7676060.39794215125534366No Hit
AAATATAGATGGATACGAATAAGGCGTCC6789410.3519764594276938No Hit
ATATAGATGGATACGAATAAGGCGTCC5887980.30524454313129895No Hit
AAGAGGTGCACAATCGACCGATCCTGAT5619690.2913358582382293No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA5558220.28814913170955886No Hit
TTTTCCACGTTCTAGCATTCAAGGTC5293780.27444003844062104No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT5276030.273519843290403No Hit
TCAATATAGATGGATACGAATAAGGCGTCC5078600.2632846811209642No Hit
TAAGAGGTGCACAATCGACCGATCCTGA4913620.25473179121206474No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA4702570.24379054127102207No Hit
ATGATTAATAGGGACGGTCGGGGGC4567780.23680276074719764No Hit
AAGAGGTGCACAATCGACCGATCCTG4357730.22591335278644892No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA4315850.22374221065173736No Hit
AGAGGTGCACAATCGACCGATCCTGA4291190.22246378741768805No Hit
ACGTTCTAGCATTCAAGGTC4287180.2222559010767092No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT4274190.2215824737526905No Hit
TCCACGTTCTAGCATTCAAGGTCCC4251780.2204206949743026No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTT4213680.2184455155251023No Hit
TCTATGCCGACTAGGGATCGGGTGGTG4207560.21812824260570318No Hit
AAGAGGTGCACAATCGACCGATC4171520.21625985763590846No Hit
ATGTATTTATTAGATAAAAAATC4151260.2152095391151526No Hit
TCACGTTCTAGCATTCAAGGTCC4064320.21070239734839472No Hit
TACGCGAAACTCAGGTGCTGCAATCT3300040.17108060864931807No Hit
TCACGTTCTAGCATTCAAGGTCCCA3239260.16792965308705046No Hit
TAGAGGTGCACAATCGACCGATCCTGA3237050.1678150823106008No Hit
TGATGTATTTATTAGATAAAAAATCAATG3035310.15735648120609497No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT2886320.14963254456209613No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGA2768820.14354111187755447No Hit
TGATGTATTTATTAGATAAAAAATCA2753790.14276192691373607No Hit
AGAGGTGCACAATCGACCGATC2593020.13442729174187426No Hit
AAGAGGTGCACAATCGACCGATCCT2574620.13347339930446517No Hit
AGAGATGTATTTATTAGATA2508550.1300481996664425No Hit
TTGCCTTGTTGTAGACGGCCTTGGT2490630.1291191913795745No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGG2427190.12583033614972497No Hit
ATGCCGACTAGGGATCGGGTGGTG2420290.12547262648569654No Hit
TATAGATGGATACGAATAAGGCGTCC2397450.12428855565578224No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA2366700.12269441476174262No Hit
TCCACGTTCTAGCATTCAAGGTCCCA2329280.1207544878591422No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT2148630.11138923411903622No Hit
ATGAGGTGCACAATCGACCGATCCTGA2117230.10976139593780551No Hit
TGTTCTAGCATTCAAGGTCCC2106310.10919528151299533No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCT2099900.10886297441931098No Hit
TGCTTGGTGGGGCTTGCTCTGCTAGGCG2063100.10695518954449282No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA2021330.10478975002761363No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCC1947720.10097366185817438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)