FastQCFastQC Report
Sun 1 Oct 2023
SRR7471282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71142190
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length20-45
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA14989722.1070085135135703No Hit
ACGTTCTAGCATTCAAGGTCCCA9834141.3823217980779057No Hit
TCCGATATAGTGTAACGGCTATCACATCACG9453591.3288303326057296No Hit
AAGAGGTGCACAATCGACCGATCCTGA9329021.3113203290480655No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAG8921521.2540406754416753No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC7940541.1161506273562847No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG7419191.0428678116318881No Hit
ACGTTCTAGCATTCAAGGTCCC7213401.0139412351517434No Hit
ACGCGAAACTCAGGTGCTGCAATCT6581330.9250952212744646No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC5686660.7993372146682579No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC5188780.7293534258644554No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA5013050.7046521902123057No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCG4932590.6933424456008452No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA4929170.692861718201253No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT4631020.6509526906607739No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCA4574290.6429785194973616No Hit
TACGCGAAACTCAGGTGCTGCAATCT2672310.37562942608317235No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC2650610.37257919667640255No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA2600720.3655664803121748No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA2587920.36376726665288206No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGC2513810.35335010069271133No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG2494240.35059927168393323No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG2423760.3406923514724526No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGC2118430.2977740775199639No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG2002540.2814841657250079No Hit
GCGTAGTCGGTAGCGCTCTCCCT1969500.2768399454669585No Hit
GTCTAGTGGTATGATTCTCGCT1872910.2632629105176548No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGC1788630.25141621307974915No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA1750050.2459932706597871No Hit
ATCGAAGGGTTGCAGGTTCATTTCCTGTCCGT1641260.23070136019147006No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG1622210.22802362423760078No Hit
ATGTATTTATTAGATAAAAAATCAATG1599790.22487218906249584No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC1552750.21826007886459495No Hit
TTGTCCGCGCGAGTTCGAACCTCGCATCCTT1496640.21037305711280466No Hit
TTGTCCGCGCGAGTTCGAACCTCGCATCCT1496540.21035900075609143No Hit
ATGTACACGGAGTCGACCCGCAACGCGA1474320.20723567829441292No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAG1463680.20574008194012583No Hit
TATACGCGAAACTCAGGTGCTGCAATCT1453740.20434288008283127No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCC1450690.20391416120307793No Hit
TCGGGTCTTGTAATTGGAATGAGTAC1323310.18600917402177244No Hit
ACTATGCCGACTAGGGATCGGGTGGTG1219250.17138212922599094No Hit
AAGCGGTGGCTCAATGGTAGAGCTTTCGACT1206520.16959275501639742No Hit
ACGTTCTAGCATTCAAGGTCC1199070.1685455564412622No Hit
ACCTGCGCAGGTTCGTATCCTGTCTGTGACG1181260.1660421193106369No Hit
TTGTCCGCGCGAGTTCGAACCTCGCATCCTTCA1144370.16085672931912834No Hit
TCACGTTCTAGCATTCAAGGTCCC1101070.1547703268623021No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCC1089470.15313978948356805No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCC1083560.15230905880181647No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC1079300.1517102580058331No Hit
TTGCAAGAGTTCGAATCTCTTAGCAACCACCA1078420.15158656206675675No Hit
TTACGCGAAACTCAGGTGCTGCAATCT1062450.14934176189965476No Hit
TAGCGTGTTACCTATACTCTACCGTCAGGGT1049870.14757347222513112No Hit
TCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1020350.14342403572338722No Hit
ATGTATTTATTAGATAAAAAATCA1013900.1425174007153842No Hit
CTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1013140.1424105724043637No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT1009310.14187221394224722No Hit
GTTTAGTGGTAAAATCCAACGT1003310.14102883253945372No Hit
TTGCCCGCGCTGGTTCAAATCCTGCTGGTGTCG974560.13698762998440167No Hit
ATCGGGCGTTCGACTCGCCCCCGGGAGA950510.13360707619487114No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG939480.13205666004940247No Hit
ACCTGCGCAGGTTCGTATCCTGTCTGTGACGC926280.13020122096325681No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT921830.12957571308951835No Hit
AGAGATGTATTTATTAGATAAAAAATCA920760.12942531007268682No Hit
GCCTTGTTGGCGCAATCGGTAGCGCTTATGA911510.12812509707671355No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA890570.12518169598096432No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT883890.12424273135252092No Hit
AGGTGGGGACACGGGTTCGATTCTCGTACCGCT863830.12142302619584806No Hit
ATCCGTGATAGTTTAATGGTCAGAATGGGCG846370.11896878631371904No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGC800920.11258017218755847No Hit
ATATATGATGATATCCTATAACAACAACA799280.11234964793746158No Hit
GTCTAGTCGGTTATGGCATCTGCT792840.11144441856512993No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT784690.11029882549300211No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG776170.10910122390103538No Hit
AGATGCAAGAGTTCGAATCTCTTAGCAACCA771700.10847290475595424No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGT758870.10666947418964752No Hit
ACCTGCGCAGGTTCGAATCCTGTCTGTGACG747490.10506986079568256No Hit
GCGTAGTCGGTAGCGCCTCCCT724290.10180878603821446No Hit
TATCTGGTTGATCCTGCCAGTAGTC724140.1017877015031446No Hit
ATCTGGTTGATCCTGCCAGTAGTC723540.10170336336286527No Hit
CAAGAGGTGCACAATCGACCGATCCTGA719590.1011481372726929No Hit
TAATGACCCACTCGGCACCTTACGAGAAATC714640.10045234761538828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)