FastQCFastQC Report
Sun 1 Oct 2023
SRR7471283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24896330
Total Bases645.4 Mbp
Sequences flagged as poor quality0
Sequence length20-45
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTCTAGCATTCAAGGTCCCA18436047.4051235664051696No Hit
ACGTTCTAGCATTCAAGGTCCC16163346.4922580958719625No Hit
ACGCGAAACTCAGGTGCTGCAATCT14613475.8697285905191645No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC12157604.883290027084313No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA11361804.563644521100098No Hit
ATGTATTTATTAGATAAAAAATCAATG10948084.3974674178885005No Hit
ATGTATTTATTAGATAAAAAATCA9597913.855150538252024No Hit
AAGAGGTGCACAATCGACCGATCCTGA7291802.9288654191200068No Hit
ACGTTCTAGCATTCAAGGTCC4794441.925761748820007No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC4105541.6490542983644576No Hit
TGATGTATTTATTAGATAAAAAATCA3753561.5076760309652066No Hit
AGAGATGTATTTATTAGATAAAAAATCA2762261.1095048948981638No Hit
TTGGAAGAGATGTATTTATTAGATA2512031.0089961050484149No Hit
TTTGGAAGAGATGTATTTATTAGATA2493371.001501024448182No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA2311250.9283496804549105No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA2248700.9032254954846759No Hit
TCCAACGGGGCCTTTCCTTCTGGC2138580.8589940766369983No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC2064490.8292346703309283No Hit
AGAGATGTATTTATTAGATAAAAAATC2046720.8220970721387449No Hit
ATGTACACGGAGTCGACCCGCAACGCGA1999910.8032951041378389No Hit
ATGTATTTATTAGATAAAAAATCAAT1828720.7345339654479194No Hit
TACGCGAAACTCAGGTGCTGCAATCT1817180.729898744112084No Hit
ATGTATTTATTAGATAAAAAATCAA1661570.6673955558911695No Hit
TTGGAAGAGATGTATTTATTAGATAA1635810.6570486493390792No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCT1406380.5648945045313908No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT1281650.5147947508729198No Hit
TTTGGAAGAGATGTATTTATTAGATAA1227190.49292004082529434No Hit
ACTATGCCGACTAGGGATCGGGTGGTG1224920.49200825985195407No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT1134960.4558744200450428No Hit
TGGAAGAGATGTATTTATTAGATA1037990.41692490419270634No Hit
ATGTATTTATTAGATAAAAAATC1022700.41078343675553786No Hit
TGATGTATTTATTAGATAAAAAATCAATG980120.39368051435693535No Hit
TGATGTATTTATTAGATAAAAAATC975770.391933268879389No Hit
TCCACGTTCTAGCATTCAAGGTCCCA882120.3543172829087661No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC867040.3482601652532723No Hit
ATTTATTAGATAAAAAATCA798340.32066573667685155No Hit
ACGTTCTAGCATTCAAGGTC797610.3203725207691254No Hit
TTTTCCACGTTCTAGCATTCAAGGTC795000.3193241734825976No Hit
AAGAGGTGCACAATCGACCGATCCT774760.3111944611916696No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT746260.2997469908215388No Hit
TGGAAGAGATGTATTTATTAGATAA709740.2850781621226904No Hit
AGAGATGTATTTATTAGATAAAAA704810.28309795058147125No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC676300.27164646355507016No Hit
TCCACGTTCTAGCATTCAAGGTCCC642590.258106315268154No Hit
TCACGTTCTAGCATTCAAGGTCCCA635450.25523842269121594No Hit
AAGAGGTGCACAATCGACCGATCCTG632780.2541659754670668No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA603640.2424614390956418No Hit
AACGGGGCCTTTCCTTCTGGCTAACCT598960.2405816439611782No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCT581290.23348421233169708No Hit
TTTCCACGTTCTAGCATTCAAGGTCC577200.23184139991717653No Hit
AGAGATGTATTTATTAGATAAAAAA538330.21622865699482616No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA519840.20880185955118685No Hit
TTCCACGTTCTAGCATTCAAGGTCCCA505590.2030781243661214No Hit
TCCAACGGGGCCTTTCCTTCTGGCT478350.19213675268603847No Hit
ATGATTAATAGGGACGGTCGGGGGC472200.18966650907985233No Hit
TTCCACGTTCTAGCATTCAAGGTCCC463670.1862403012813535No Hit
TCGTATGGCGCAGTGGTAGCGCAGCAGA452910.18191837913459535No Hit
TTGGAAGAGATGTATTTATTAGATAAA444320.1784680713984752No Hit
ACGGGGCCTTTCCTTCTGGCTAACCT409180.16435354126491736No Hit
CAAGAGGTGCACAATCGACCGATCCTGA396970.15944920395897708No Hit
AGATGTATTTATTAGATAAAAAATCA376900.15138777482464283No Hit
CCAGATCGGGGTTCAATTCCCCGTCGCGGA368480.14800575024511645No Hit
ATTACCCAATCCTAATTCAGGGAGGTAG351970.141374250742981No Hit
TTACGCGAAACTCAGGTGCTGCAATCT348030.13979168817251378No Hit
TAGAGATGTATTTATTAGATAAAAAATCA345410.13873932422971577No Hit
ATTTCCACGTTCTAGCATTCAAGGTCCCA337540.13557821574505158No Hit
TCACGTTCTAGCATTCAAGGTCCC333760.1340599196749079No Hit
TTTGGAAGAGATGTATTTATTAGATAAA328680.13201945828963546No Hit
ATTTCCACGTTCTAGCATTCAAGGTCCC320650.1287940833046477No Hit
TTGATGTATTTATTAGATAAAAAATCA301240.12099775348414807No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTT299880.12045148823139795No Hit
AAGAGGTGCACAATCGACCGATCCTGAT297980.1196883235400559No Hit
ATTTATTAGATAAAAAATCAATG294920.11845922672136816No Hit
TAGAGATGTATTTATTAGATAAAAAATC290420.11665173139976856No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACC287970.11566765061356434No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA282140.11332594000802527No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGC282080.11330184007040396No Hit
TGATGTATTTATTAGATAAAAAATCAA277940.1116389443745323No Hit
TGCTTGGTGGGGCTTGCTCTGCTAGGCG270980.10884335161045824No Hit
AGAGGTGCACAATCGACCGATCCTGA268650.10790747069949666No Hit
TTGGGTACAAATGGCAGTCTGACAAGT268230.10773877113614738No Hit
TTGGCGTCGCTGAACCATAGCAGGCT264460.10622449172227394No Hit
TCTATGCCGACTAGGGATCGGGTGGTGT263000.1056380599068216No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCC258830.1039631142421393No Hit
AGAGATGTATTTATTAGATAAAA255930.10279828392377512No Hit
TCGCGAAACTCAGGTGCTGCAATCT254540.10223996870221434No Hit
TTCTAGCATTCAAGGTCCCA253360.10176600326232822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)