FastQCFastQC Report
Sun 1 Oct 2023
SRR7471284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51801884
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length20-45
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA881726217.021122243353158No Hit
ACGCGAAACTCAGGTGCTGCAATCT5468281.0556141162742265No Hit
ACGTTCTAGCATTCAAGGTCCCA5224601.008573356135078No Hit
ACGTTCTAGCATTCAAGGTCCC3171550.6122460719768417No Hit
ACGGAGTCGACCCGCAACGCGA2870090.5540512773628079No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA2778410.5363530793590442No Hit
TCCAACGGGGCCTTTCCTTCTGGC2644930.5105856767680497No Hit
ATGTATTTATTAGATAAAAAATCA2476380.4780482501370027No Hit
ATGTATTTATTAGATAAAAAATCAATG2387030.4607998427238669No Hit
TACGCGAAACTCAGGTGCTGCAATCT2213960.4273898609556363No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT2169470.4188013702358779No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC2139380.4129927011921034No Hit
ACACGGAGTCGACCCGCAACGCGA2122720.40977660194752763No Hit
AAGAGGTGCACAATCGACCGATCCTGA1656540.31978373605099No Hit
ATATATGATGATATCCTATAACAACAACA1534390.2962035125981132No Hit
AAACATAGCATTGCGATGGTCA1528270.2950220883858201No Hit
AAACATAGCATTGCGATGGTCAG1468820.28354567181378965No Hit
AGAGATGTATTTATTAGATAAAAAATCA1430750.27619651825790736No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCC1234830.23837550001077182No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA1224740.2364276944058637No Hit
TATACGCGAAACTCAGGTGCTGCAATCT1188450.22942215769604057No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG1014770.19589441959292445No Hit
TACACGGAGTCGACCCGCAACGCGA1011840.1953288030991305No Hit
ATGTACACGGAGTCGACCCGCAACGC990890.19128454864691793No Hit
ATATATGATGATATCCTATAACAACA989890.1910915054749746No Hit
AAAACATAGCATTGCGATGGTCAG981040.18938307340327623No Hit
AACTTATGTTGCCTTCCTTCTGAAA970940.1874333373666487No Hit
ACGGGCTTGGCAGAATCAGCGGGGA910960.17585460791348823No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT879280.16973900022632382No Hit
ATATATGATGATATCCTATAACAACAAC877620.16941854856089789No Hit
AAAACATAGCATTGCGATGGTCA860410.16609627557175335No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA859310.16588392808261568No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA849010.16389558341159946No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC847220.1635500361338209No Hit
ATACGCGAAACTCAGGTGCTGCAATCT808180.15601363070115365No Hit
ACGTTCTAGCATTCAAGGTCC750560.14489048313377945No Hit
ATGTATTTATTAGATAAAAAATC746270.1440623279261426No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCA730470.1410122458094381No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA721110.13920536172004863No Hit
TGATGTTGACGCAATGTGATTTCTGCCCA696670.13448738659775386No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGC695150.13419396097640002No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCC685970.13242182465796032No Hit
ATGTACACGGAGTCGACCCGCAACGCG670590.12945282067347202No Hit
TCCGATATAGTGTAACGGCTATCACATCACG668720.12909182994193802No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC644570.1244298373395068No Hit
ATTAAGCCTTTGTTGTCTGATTTGT639800.12350902140933716No Hit
TGATGTATTTATTAGATAAAAAATCA639630.12347620407010679No Hit
AGAGATGTATTTATTAGATAAAAAATC596800.11520816501577433No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC584930.1129167425648071No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT568880.10981839965511679No Hit
ACTATGACTCTCTTAAGGTAGCCAAATGCC546800.10555600641860825No Hit
TCGAAATGAAGATAAAAATTTCCATCGA527350.10180131672431064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)