FastQCFastQC Report
Sun 1 Oct 2023
SRR7471288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7529882
Total Bases384 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG2935763.898812756959538No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT2010612.6701746454990927No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA1787862.374353276718015No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT1086401.4427848935746934No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1032501.3712034265610007No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG913041.2125555221184077No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC845931.1234306194970918No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT705520.9369602339053918No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT655160.8700800357827653No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA514120.6827729836935028No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT456240.6059059092825093No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA404880.5376976691002595Illumina Single End Adapter 1 (95% over 22bp)
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT397540.5279498403826248No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG362440.48133556408984896No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG348650.4630218640876444No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG339740.45118900933640127No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT336220.4465143012865274No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG330270.43861245103176916No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC288430.3830471712571326No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT288260.3828214041069966No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG278840.3703112479053457No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT277580.36863791491022035No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA270540.3592884988104727No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA234930.31199692106728893No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT204430.27149163824878003No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT204120.27107994521029677No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT183070.24312465985522747No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC171850.22822402794625468No Hit
AGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGT155040.20589964092398794No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT151880.2017030280155785No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT144210.19151694541826816No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT144100.19137086079170962No Hit
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTATG144000.19123805658574727No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG141160.18746641713641726No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT132870.17645694846214058No Hit
TTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTAT127960.16993626194939043No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA126480.16797075970114803No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA125950.16726689740954773No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA124950.16593885534992447No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG124900.16587245324694333No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTC119810.159112719163461No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC119360.15851510023663054No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT119040.1580901267775511No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAATTC117700.15631055041765593No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT116090.15417240270166252No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC115720.1536810271396019No Hit
AGAGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCG112930.14997578979325307No Hit
TTTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTA112170.1489664778279394No Hit
TAGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTA106820.14186145280895504No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAAT104860.13925849037209348No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA100780.13384007876883064No Hit
TTGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCG96910.1287005559980887No Hit
ATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCCGTCT95550.12689441879700106No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAAT93670.12439769972490936No Hit
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG93030.12354775280675048No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA92420.12273764715038031No Hit
TGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGT88960.11814262162408387No Hit
TGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTA85980.11418505628640661No Hit
GAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTAT82640.10974939580726498No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTCTGTAGGCACCATCAATTC80550.1069737879026524No Hit
TTGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTAT78180.10382632822134531No Hit
AAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT77900.10345447644465079No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)