FastQCFastQC Report
Sun 1 Oct 2023
SRR7471289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7597431
Total Bases387.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG2918293.84115367418276No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT2144212.8222829532772327No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA1787952.353361287519426No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT1103001.4518065382890613No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1047061.3781763862021255No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG909511.1971283450945458No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC848061.1162457414881426No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT698730.9196924592010115No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT670180.8821139672081261No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA545890.7185191941855082No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT451370.5941087191183441No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA409670.539221744824007Illumina Single End Adapter 1 (95% over 22bp)
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT393710.5182146438710664No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG368710.4853087839823751No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG359370.47301515472796No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG348980.4593394793582199No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG348630.4588787973197782No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT344150.4529820672277247No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT307530.40478156366276963No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG290840.38281361160107935No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC286230.37674577103760465No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT280800.3695986182697809No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA267520.3521190254969081No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA247080.3252151944519141No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT204100.26864344013127595No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT202330.2663137052511566No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT182430.24012064077975828No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC167970.22108789142013924No Hit
AGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGT155650.2048718836669922No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT153400.20191035627700996No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT151180.19898831591889415No Hit
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTATG147210.19376286536857No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT145880.19201227362249162No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT142720.187852972932561No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG141200.1858522966513286No Hit
TTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTAT134110.17652019478689573No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC125790.16556912461593926No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA125700.16545066352033996No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA125390.1650426308577202No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA124890.16438451365994636No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT124100.16334468848746372No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTC123210.1621732398754263No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG122600.16137033689414226No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAATTC118310.15572369133724281No Hit
TTTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTA116170.15290694973077085No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC115130.15153806595940128No Hit
AGAGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCG114950.1513011437682027No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT114820.1511300332967815No Hit
TAGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTA112040.147470901677159No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAAT107100.14096870376315362No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT105290.13858631950721237No Hit
TTGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCG102460.13486137616781252No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA100120.131781387682231No Hit
ATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCCGTCT97490.1283196912219407No Hit
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG95930.12626636556488632No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA94440.12430517631552034No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAAT93760.12341013692654794No Hit
TGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGT91770.1207908304794081No Hit
TGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTA84520.11124813111168762No Hit
TTGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTAT83430.10981343562054068No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTCTGTAGGCACCATCAATTC81780.10764164886788706No Hit
GAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTAT80520.10598319352949702No Hit
AAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT79250.10431157584715148No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAA78500.10332440005049075No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)