FastQCFastQC Report
Sun 1 Oct 2023
SRR7471291.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471291.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7407242
Total Bases377.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG2373183.2038645422952294No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATC2270283.064946440254011No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCAT2019262.726061872961623No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA1627212.1967825541544346No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA1240601.6748473993424273No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1045841.4119155280737419No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC914581.2347105710870525No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG865681.1686940969391846No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT764431.0320035446391518No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAA724080.9775298282410647No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG708180.9560643489169114No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCAAT535650.7231436477976553No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT510400.6890553866067829No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA502150.6779176379008542No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAAT455360.614749727361412No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATT394730.5328973995989331No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT392810.5303053417182806No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT385770.5208011294892215No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT383120.5172235496018627No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG334530.4516255847993086No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT283280.3824365398079339No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA249420.3367245190585106Illumina Single End Adapter 1 (95% over 22bp)
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT235070.31735158646092565No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCATCAA224080.3025147551544826No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCAT222150.2999091969723684No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA218720.29527859357099445No Hit
AAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT190640.257369747066452No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCAT185020.24978257764495881No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT181360.244841467309965No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATC176090.23772680843963245No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT170010.22951862515089963No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC165720.22372699582381672No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCA158150.2135072676172859No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAATTC156320.211036712449789No Hit
AGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGT153670.20745913256243012No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAATCTGTAGGCACCATCAA147910.1996829589204727No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG144760.19543036396002722No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC140290.18939572920663317No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT135020.18228107033630062No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCATC130640.17636793829606215No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG130510.17619243437705964No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATT130350.17597642955367193No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATC125380.1692667797271913No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT120440.16259763080509587No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATTCGT118820.16041058196829536No Hit
ATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCCGTCT116830.15772402197741076No Hit
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG116740.15760251926425517No Hit
AGAGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCG111290.15024485496761142No Hit
TTTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTA109270.14751779407234164No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT108100.14593825880131903No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCA104370.14090264635609312No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA102360.13818908576228509No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA100480.13565102908747953No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCA99630.13450350346323234No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG98960.13359898326529632No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA98230.1326134612585899No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA97620.13178994286942428No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC91810.1239462677201582No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAAT91470.12348725747045931No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTC88250.11914016039978172No Hit
ATGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTC85020.1147795630276424No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCAT84800.1144825563954843No Hit
ACGTTCTAGCATTCAAGGTCCCATCTGTAGGCACCATCAATTCGTATGCC82500.11137748705928603No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAACTGTAGGCACCATCAAT82330.11114798193443659No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA82310.11112098133151313No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT77650.1048298408503462No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT76770.10364181432171381No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCCTGTAGGCACCATCA76140.10279129532962471No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT75520.10195427663899734No Hit
TAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCG75410.1018057733229183No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)