FastQCFastQC Report
Sun 1 Oct 2023
SRR7471292.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471292.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7365585
Total Bases375.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG2469523.3527818903725906No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATC2323383.154372666936842No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCAT2074542.816531205600098No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA1668172.2648167117751No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA1269991.7242214976814467No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1066981.4486018422161988No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC932211.2656292745246984No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG881221.1964019151228313No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT758481.0297620623480688No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAA717980.9747766131271312No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG698630.9485057873882387No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCAAT548740.7450053186542549No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA508980.6910245418388355No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT506870.6881598678176953No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAAT457030.6204938236406206No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT406000.5512121576222391No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATT404180.5487412065708291No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT398280.5407309806349393No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT382170.518858990833722No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG346440.4703496056321392No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT291360.395569394691664No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA262740.35671301057553473Illumina Single End Adapter 1 (95% over 22bp)
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT239810.325581742658594No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCATCAA226020.3068595366152179No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA223140.3029494602261735No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCAT218180.2962154397783747No Hit
AAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT194690.2643238792302309No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCAT186130.25270226329612655No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT183950.24974255269608592No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATC173900.2360980152968162No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT171750.2331790346591615No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC170480.2314547995848259No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAATTC161530.21930369413970513No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCA159040.21592310726167713No Hit
AGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGT155760.21146996470748758No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAATCTGTAGGCACCATCAA149620.2031338990725109No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG145620.19770323742106022No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC145290.19725520783481554No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT137870.18718133047137464No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCATC137260.18635315456952842No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATT133950.18185928205295304No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG131170.17808497220519484No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATC125810.170807885592251No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATTCGT121150.164481164768311No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT119210.16184729386735747No Hit
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG117530.15956641597374818No Hit
ATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCCGTCT115210.15641663221590682No Hit
AGAGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCG114650.1556563395847037No Hit
TTTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTA111110.15085020402316993No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT109930.14924815883599196No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA105500.14323370105701042No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCA102630.13933720132209457No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA102000.13848187211199112No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA100900.13698844015784217No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG99240.13473471557249017No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCA99010.13442245252753177No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA93820.12737616903477456No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAAT92130.12508171448703667No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC91710.12451149501363436No Hit
ATGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTC88980.1208050684365193No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTC88860.12064214858697579No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCAT86440.11735659828784814No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA85630.1162568893034294No Hit
ACGTTCTAGCATTCAAGGTCCCATCTGTAGGCACCATCAATTCGTATGCC84360.11453265422909381No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAACTGTAGGCACCATCAAT84170.11427469780064992No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCCTGTAGGCACCATCA79530.10797513028496719No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT79120.10741848746569349No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT77850.1056942523913579No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT77040.10459454340693917No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT74070.10056227713073707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)