FastQCFastQC Report
Sun 1 Oct 2023
SRR7471293.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471293.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7491858
Total Bases382 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG2487693.320524761681281No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATC2377933.1740190484122897No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCAT2107842.813507677267775No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA1699312.2682090343944052No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA1308601.7466962134092772No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1106471.4768966523391127No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC958651.2795891219507898No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG900401.2018380487190228No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT748520.9991113018960049No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAA715900.9555707008862154No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG676820.9034074057463448No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCAAT558560.7455560423061943No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT532290.7104913093654471No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA520500.6947542251868628No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAAT474020.6327135404862185No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT411940.5498502507655645No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATT411870.549756815999449No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT405120.5407470349811755No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT396740.5295615586947857No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG350640.4680280912959109No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT295310.39417458259353017No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA258010.34438720007773777Illumina Single End Adapter 1 (95% over 22bp)
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT245280.32739542046846054No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCATCAA231710.3092824236657982No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCAT228410.30487764183464233No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA222200.2965886432978308No Hit
AAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT199690.2665426920798552No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT190950.2548766941391575No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCAT184740.24658769560234592No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATC183740.24525291322926834No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT175630.2344278281836095No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC172310.22999635070499203No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCA165700.22117343921894944No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAATTC165400.22077300450702614No Hit
AGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGT157970.21085557147506No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG150370.20071122543967065No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAATCTGTAGGCACCATCAA149430.19945653000897776No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC148850.19868235623259276No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT141100.18833779284124177No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATT139100.18566822809508668No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCATC138580.18497414126108636No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG138270.18456035872543233No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATC131370.17535036035119725No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATTCGT122390.1633640146409609No Hit
AGAGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCG116180.15507501610414934No Hit
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG115370.1539938423819565No Hit
ATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCCGTCT114840.15328640772422542No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT113120.15099058204253202No Hit
TTTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTA107960.1441031049974519No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA106370.14198080102425858No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCA103530.13819001908471837No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG101710.13576071516571722No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA101400.1353469326300632No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA100720.13443928061637045No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCA100580.1342524110841396No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA99830.13325132430433145No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC94400.12600345601852037No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAAT93740.1251224996522892No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTC93150.12433497805217344No Hit
ATGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTC87880.11730067494605477No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA87070.11621950122386196No Hit
ACGTTCTAGCATTCAAGGTCCCATCTGTAGGCACCATCAATTCGTATGCC86490.11544532744747699No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCAT85650.11432411025409184No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAACTGTAGGCACCATCAAT85520.11415058854559176No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCCTGTAGGCACCATCA80970.10807732874808892No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT80190.10703619849708843No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT79940.10670250290381905No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT79510.1061285464833957No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATT76460.10205746024550919No Hit
TAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCG75110.1002555040418545No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)