FastQCFastQC Report
Sun 1 Oct 2023
SRR7471295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7593116
Total Bases387.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG2500293.2928378810490972No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATC2392013.150235028675975No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCAT2110332.7792674311837193No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA1703642.243663866059731No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA1311631.727393602310303No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1117211.4713458875117935No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC968731.2758003433636467No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG902041.187970788277171No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT782991.0311840356449185No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAA764751.0071622769887882No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG715030.9416819129327143No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCAAT569240.7496790513933936No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT552350.727435218953589No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA521130.6863190289730856No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAAT487640.6422132889843906No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATT415970.5478251616332478No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT413340.5443614979673694No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT410180.5401998336387854No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT405730.5343392620368239No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG348910.4595083230652607No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT298530.393158750636761No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA260910.34361387340849264Illumina Single End Adapter 1 (95% over 22bp)
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT241980.3186833969084629No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCAT240870.31722154646392864No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA238260.31378422244569953No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCATCAA231050.3042887794681393No Hit
AAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT202630.26686014015853304No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCAT199120.262237531996087No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATC190750.2512143894548694No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT190080.250332011258619No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT180810.23812358457318444No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC172760.22752187639435512No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCA171710.22613904489276865No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAATTC166380.21911952879423943No Hit
AGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGT159000.20940019881166044No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG157590.20754325365238724No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAATCTGTAGGCACCATCAA157580.2075300838285626No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC147670.1944787884183516No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT140940.18561549698437374No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG140790.18541794962700425No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCATC138020.18176990842758098No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATT137920.18163821018933465No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATC137040.18047926569276698No Hit
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG125770.1656368742424059No Hit
ATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCCGTCT123430.16255513546744182No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATTCGT122910.16187030462856092No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT119620.15753743259025677No Hit
AGAGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCG116600.15356014579521768No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT115350.15191391781713856No Hit
TTTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTA114140.150320369134358No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCA110710.145803119562509No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA108120.14239213519192911No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA107490.14156243629097726No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCA104700.1378880554439047No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA102260.1346746184306943No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA100940.1329362016858428No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG100040.13175091754162588No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAAT96940.12766827215598972No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC96530.12712830937917977No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTC95440.12569279858229482No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCAT92030.12120188865809504No Hit
ATGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTC89870.11835720671197437No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAACTGTAGGCACCATCAAT87830.11567056265174928No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA87740.1155520342373276No Hit
ACGTTCTAGCATTCAAGGTCCCATCTGTAGGCACCATCAATTCGTATGCC87440.11515693952258861No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT84940.11186448356643043No Hit
TAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCG81870.1078213476522682No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT81430.10724187540398435No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCCTGTAGGCACCATCA81110.10682044104159609No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATT78390.10323824896129599No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT78190.10297485248480333No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)