FastQCFastQC Report
Sun 1 Oct 2023
SRR7471296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7825038
Total Bases399 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG2472463.1596779466118887No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATC2394033.0594484014007346No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCAT2122852.7128941738046515No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA1714782.1914014986253103No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA1326691.6954422457756757No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1120181.4315329842487667No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT1047501.3386516461645297No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC970121.239763947472204No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAA964531.2326202121957746No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG957591.2237512456808517No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG901111.1515726824585388No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT593050.7578876933249398No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCAAT563110.7196258983023469No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA529930.6772235483073693No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAAT529730.6769679584942591No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT438000.5597416907112783No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT413270.5281380103202055No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATT412190.5267578253294105No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT400740.5121253085288532No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG346510.4428221307040298No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT301300.38504605345047527No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA293290.3748096814354128No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCAT257290.32880351507558175No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA253620.3241134420050101Illumina Single End Adapter 1 (95% over 22bp)
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCAT248970.31817097885019857No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT244340.3122540746766981No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCATCAA234140.29921899420807924No Hit
AAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT205780.2629763587090567No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATC204650.26153227626498426No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT189390.2420307735246781No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCA186260.2380307929495039No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT177700.22709154894838848No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC170940.21845261326526466No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG170230.21754526942872354No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAATTC166290.21251015011045316No Hit
AGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGT163630.20911080559608783No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT161950.2069638511659624No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAATCTGTAGGCACCATCAA160120.20462520437600432No Hit
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG157340.2010725059737729No Hit
ATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCCGTCT154950.19801820770710635No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC148000.18913646170152784No Hit
TTTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTA147350.18830579480891976No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATC146930.18776905620138842No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG143300.18313010109343877No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT141930.1813793108736341No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA141040.18024193620529383No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATT139530.17831223311631203No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCA137000.17507902198046835No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCCTGTAGGCACCATC135320.1729320675503429No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCA134240.17155188255954795No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAAT127470.16290016738576862No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATTCGT125400.1602548128200783No Hit
AGAGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCG118600.15156475917433243No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATT118270.15114303598270065No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCAT114830.1467468911972057No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA112860.1442293315380705No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT107680.13760955537851702No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA106120.13561595483625766No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG103580.13236996420975847No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTC101520.1297373891347237No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA98980.1264913985082245No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC98250.12555849569037236No Hit
TAAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCG93470.11944989915703924No Hit
ATGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTC91760.11726460625494727No Hit
TTTGGAAGAGATGTATTTATTAGATAAAACTGTAGGCACCATCAATTCGT90830.11607611362398497No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAACTGTAGGCACCATCAAT89940.1149387389556447No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA88930.11364801039943832No Hit
ACGTTCTAGCATTCAAGGTCCCATCTGTAGGCACCATCAATTCGTATGCC86380.1103892402822836No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATT83620.10686210086136322No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT82130.10495795675369245No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAA80340.10267042792635639No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT79240.10126468395425044No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCCTGTAGGCACCATCA79180.10118800701031738No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)