FastQCFastQC Report
Sun 1 Oct 2023
SRR7471297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16750865
Total Bases854.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA464967727.75783220747108Illumina Single End Adapter 1 (95% over 22bp)
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC3731362.2275625766191776Illumina Single End Adapter 1 (95% over 22bp)
ACCNGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3672932.192680795887257Illumina Single End Adapter 1 (95% over 22bp)
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1801911.075711612504787Illumina Single End Adapter 1 (95% over 22bp)
CCTNTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1666800.9950530912881216Illumina Single End Adapter 1 (95% over 22bp)
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1403130.8376462946838865Illumina Single End Adapter 1 (95% over 22bp)
ATGNACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1037950.6196396424901042No Hit
ACANGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA857410.5118601337901058Illumina Single End Adapter 1 (95% over 22bp)
ACTNTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA667710.39861225077033335Illumina Single End Adapter 1 (95% over 22bp)
CTANAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA609510.3638677763805033Illumina Single End Adapter 1 (95% over 22bp)
CAGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA533640.31857459301355484Illumina Single End Adapter 1 (95% over 22bp)
CATNTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA488480.2916147912361541Illumina Single End Adapter 1 (95% over 22bp)
AAANGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA459480.27430225245084355Illumina Single End Adapter 1 (95% over 22bp)
AACNGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA349590.20869967013643775Illumina Single End Adapter 1 (95% over 22bp)
TTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA315280.18821714580112728Illumina Single End Adapter 1 (95% over 22bp)
CTCNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA258880.1545472427841786Illumina Single End Adapter 1 (95% over 22bp)
CTGNAGGCACATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA250890.14977733985677755Illumina Single End Adapter 1 (95% over 22bp)
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAACAAA231600.13826151664406583Illumina Single End Adapter 1 (95% over 22bp)
CTGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA221190.13204691220423542Illumina Single End Adapter 1 (95% over 22bp)
CCGNAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA208290.12434581736525249Illumina Single End Adapter 1 (95% over 22bp)
CTGNAGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA174690.10428715173813412Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)