FastQCFastQC Report
Sun 1 Oct 2023
SRR7471298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9504919
Total Bases276.6 Mbp
Sequences flagged as poor quality0
Sequence length20-45
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATATAGTGTAACGGCTATCACATCACGC1675001.762245422606968No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT1585571.6681572983420478No Hit
ATGTACACGGAGTCGACCCGCAACGCGA924250.972391242892233No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCT648850.6826465328110635No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCC554170.5830349527439425No Hit
ACGCGAAACTCAGGTGCTGCAATCT519190.5462329557989921No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGC480670.5057065715131291No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG447200.4704932256655738No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGT413790.4353430050271864No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT388990.4092512519044087No Hit
AGATGCAAGAGTTCGAATCTCTTAGCAACCACCA387560.407746767752571No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC315710.33215432977387815No Hit
TCCGATATAGTGTAACGGCTATCACATCACG315290.33171245330970206No Hit
ACGTTCTAGCATTCAAGGTCCCA292090.30730403909807125No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCC286440.3013597485680835No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCC277820.29229076018427935No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT252260.26539942107870673No Hit
ATATATGATGATATCCTATAACAACAACA251840.26495754461453064No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTT244130.2568459552364412No Hit
ACCTGCGCAGGTTCGTATCCTGTCTGTGACGCCA241320.2538895912737394No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGC238580.2510068733884002No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCT234530.24674592176955953No Hit
TAGTGTAACGGCTATCACATCACGC217970.22932336403918854No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGT206620.21738217863823983No Hit
ATGCAAGAGTTCGAATCTCTTAGCAACCACCA205570.21627748747779968No Hit
GTCTAGTGGTATGATTCTCGCT200540.21098549077588144No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGC188120.19791857247810318No Hit
GCGTAGTCGGTAGCGCTCTCCCT186250.19595117012570018No Hit
TATACGCGAAACTCAGGTGCTGCAATCT164810.17339442871633098No Hit
ACCTGCGCAGGTTCGAATCCTGTCTGTGACGCCA159940.16827076590552745No Hit
TACCATACGCGAAACTCAGGTGCTGCAATCT156830.16499877589698556No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG155420.1635153334815373No Hit
ACAACACATGCAGATCTGAGCCTT153080.16105345032398488No Hit
GCCTTGTTGGCGCAATCGGTAGCGCTTATGA152320.16025386434119007No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCA148540.1562769761636054No Hit
GCCCAGTTGGTTAAGGCACCGTGC146530.15416228165647702No Hit
ACGGGTTCGATTCTCGTACCGCTCACCA143610.15109018814363384No Hit
TACAACACATGCAGATCTGAGCCTT140090.14738684253911052No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAAT134830.14185286586871493No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCA127620.13426731990035895No Hit
TTGTCCGCGCGAGTTCGAACCTCGCATCCTTCAC125670.13221575060239862No Hit
TACGCGAAACTCAGGTGCTGCAATCT124600.13109001770556908No Hit
ACGGGCTTGGCAGAATCAGCGGGGA123870.1303219943273583No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA122140.12850188412968064No Hit
ATAGTGTAGTGGTTATCACTTTCGGT115520.12153706938481013No Hit
ATTAAGCCTTTGTTGTCTGATTTGT113270.1191698740410097No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT111970.1178021611757028No Hit
ACCTGCGCAGGTTCGGATCCTGTCTGTGACGCCA110890.11666590741067863No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGTT110880.11665538654248395No Hit
ATCAAACAGTTATCCCTGTCTGAATGGGTA110230.1159715301098305No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCG108860.11453017116716092No Hit
AGGTGGGGACACGGGTTCGATTCTCGTACCGCTCACCA108530.11418298251673686No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGC107910.1135306886886674No Hit
GAAGCGGTGGCTCAATGGTAGAGCTTTCGACT106470.11201568366863515No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCA104680.1101324482617895No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGC103230.10860692237356258No Hit
ATGCCTGTTTGAGCGTCATTT102770.10812296243660784No Hit
TAAAACATAGCATTGCGATGGTCAG99540.10472472200972992No Hit
ACGTTCTAGCATTCAAGGTCCC96220.10123179376909996No Hit
AACTTATGTTGCCTTCCTTCTGAAA96010.10101085553701195No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGC95420.1003901243135265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)