FastQCFastQC Report
Sun 1 Oct 2023
SRR7471303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5061670
Total Bases258.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT94990.1876653357488734TruSeq Adapter, Index 2 (97% over 48bp)
TCGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT93160.18404992818575688TruSeq Adapter, Index 2 (97% over 48bp)
TCGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT88410.17466567358203913TruSeq Adapter, Index 2 (100% over 48bp)
CCGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT87860.17357907568055603TruSeq Adapter, Index 2 (100% over 48bp)
CTGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT87160.17219613289685026TruSeq Adapter, Index 2 (97% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT84640.16721753887550947TruSeq Adapter, Index 2 (100% over 48bp)
TTGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT69480.13726694944553872TruSeq Adapter, Index 2 (100% over 48bp)
TTGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT57780.1141520486321708TruSeq Adapter, Index 2 (97% over 48bp)
ACGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT57660.11391497272639267TruSeq Adapter, Index 2 (97% over 48bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)