FastQCFastQC Report
Sun 1 Oct 2023
SRR7471304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4924756
Total Bases251.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT90400.18356239375108127TruSeq Adapter, Index 2 (97% over 48bp)
CCGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT89310.1813490861273127TruSeq Adapter, Index 2 (97% over 48bp)
TCGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT86650.1759478033023362TruSeq Adapter, Index 2 (100% over 48bp)
CCGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT84250.17107446541513935TruSeq Adapter, Index 2 (100% over 48bp)
CTGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT83440.1694297138782104TruSeq Adapter, Index 2 (97% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT79910.16226184606912505TruSeq Adapter, Index 2 (100% over 48bp)
TTGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT68390.1388698242105802TruSeq Adapter, Index 2 (100% over 48bp)
TTGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT55720.11314266128108683TruSeq Adapter, Index 2 (97% over 48bp)
ACGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT54660.11099027038090821TruSeq Adapter, Index 2 (97% over 48bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)