FastQCFastQC Report
Sun 1 Oct 2023
SRR7471305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084891
Total Bases259.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT96250.1892862600201263TruSeq Adapter, Index 2 (97% over 48bp)
TCGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT93800.18446806431052307TruSeq Adapter, Index 2 (97% over 48bp)
TCGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT89940.17687694780478086TruSeq Adapter, Index 2 (100% over 48bp)
CCGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT88230.17351404386052718TruSeq Adapter, Index 2 (100% over 48bp)
CTGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT86900.17089845190388547TruSeq Adapter, Index 2 (97% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT83730.164664296638807TruSeq Adapter, Index 2 (100% over 48bp)
TTGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT69830.1373284107761602TruSeq Adapter, Index 2 (100% over 48bp)
TTGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT58770.11557769871566569TruSeq Adapter, Index 2 (97% over 48bp)
ACGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT56260.11064150637643953TruSeq Adapter, Index 2 (97% over 48bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)