FastQCFastQC Report
Sun 1 Oct 2023
SRR7471306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5013211
Total Bases255.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT93290.18608831744763984TruSeq Adapter, Index 2 (97% over 48bp)
TCGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT93290.18608831744763984TruSeq Adapter, Index 2 (97% over 48bp)
TCGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT89180.1778899790972293TruSeq Adapter, Index 2 (100% over 48bp)
CCGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT86080.17170631756772256TruSeq Adapter, Index 2 (100% over 48bp)
CTGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT85130.1698113245183576TruSeq Adapter, Index 2 (97% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT83540.16663970457257835TruSeq Adapter, Index 2 (100% over 48bp)
TTGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT68570.13677860357363775TruSeq Adapter, Index 2 (100% over 48bp)
TTGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT57160.11401873968600164TruSeq Adapter, Index 2 (97% over 48bp)
ACGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCT56480.11266232360856146TruSeq Adapter, Index 2 (97% over 48bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)