FastQCFastQC Report
Sun 1 Oct 2023
SRR7471307.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471307.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44511130
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT645965814.51245564873325No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG35458257.966153633933805No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT8889061.9970420881249253No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC6256421.4055855243396427No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG5709471.2827061456314408No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA5383541.2094817633252626No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTA5068321.1386635207868234No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG4308770.9680208073800868No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4144470.9311086912419433Illumina Single End Adapter 1 (95% over 22bp)
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA3791360.8517779710378056No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATGC3241800.7283122221341044No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATTCG3115640.6999687493891977No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT2995890.673065365898372No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATGC2556550.5743619629517381No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT2244040.5041525568998135No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA2015840.45288448080289134No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC1981190.4450999109660887No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1959770.4402876314306107No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCC1907440.4285310213423025No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT1811170.4069027229818699No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT1793960.4030362742981362No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1684460.378435685636379No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGT1681630.3777998896006459No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT1680430.3775302941084623No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT1659600.3728505656899746No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATTCGTATGCCG1621570.36430663521685475No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA1611060.3619454280311464No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATTCGTAT1533530.3445273126069817No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1474300.3312205284386175No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTA1445030.3246446450584382No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT1425500.3202569784231495No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1316700.2958136537984994No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGT1223440.27486159079762745No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG1204910.2706985870724917No Hit
TTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATTCGTATGCC1203390.27035709944905917No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT1199180.2694112685973149No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT1135550.25511596762427735No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC1097520.24657203715115747No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATT1083490.2434200165217104No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATG1073690.2412183200022107No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC958320.21529896005785518No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATTCGTATGC955760.2147238230078634No Hit
TTGCCTTGTTGTAGACGGCCTTGGTCTGTAGGCACCATCAATTCGTATGC898490.20185737814339919No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT895550.20119686918754928No Hit
TTGTGGCGTCGCTGAACCATAGCACTGTAGGCACCATCAATTCGTATGCC843570.1895188911177946No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATTCGTATGC815990.18332268805577392No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT798260.17933941465876063No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTATG787580.17694001477832624No Hit
ATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGTATGC765460.17197047120574113No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATTCGTATGCC703040.15794701235398875No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCCTGTAGGCACCATCAATTC696640.15650916972900936No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT678730.1524854570081685No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTC663160.14898745549708578No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA660880.14847522406193686Illumina Single End Adapter 1 (95% over 22bp)
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTA654160.1469654893057085No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATTCGTATG650300.14609829047251777No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG642570.14436164617703484No Hit
TTGCCTTGTTGTAGACGGCCTTGGCCTGTAGGCACCATCAATTCGTATGC641180.1440493647319221No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATGCCG595840.13386314838558358No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATTCGTA594500.13356210008597852No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA590890.13275106698032604No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATG590230.13260278945962503No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC584240.13125705862780837No Hit
AAGAGGTGCACAATCGACCGATCCTGCCTGTAGGCACCATCAATTCGTAT544500.12232895457832682No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG543130.12202116639141716No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA520240.11687863237801423No Hit
TTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATTCGTATGCCG504230.11328177918646415No Hit
TCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT499240.1121607112648005No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT495700.11136540456285876No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTCTGTAGGCACCATCAATTCGTA479730.10777753788771482No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG459580.1032505802481312No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGT449920.10108033653605289No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCG449540.10099496463019472No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC447140.10045577364582747No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCC445850.10016595849173003No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT445570.1001030528768872No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)