FastQCFastQC Report
Mon 2 Oct 2023
SRR7471308.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471308.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56355327
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT688486212.216878805441054No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG592735610.517827356409448No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA31836345.6492157342996165Illumina Single End Adapter 1 (95% over 22bp)
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG15015272.6643923120169277No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG13582772.4102016123515706No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATGC8796971.5609828685760265No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT7562171.3418731471472076No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTA7374991.3086588957242675No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATGC7201761.2779200092300058No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA6175431.0958023542299737No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTA5471220.9708434483930862No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA5117270.9080366085002045No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGT4823840.8559687711509507No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4640370.823412842587179Illumina Single End Adapter 1 (95% over 22bp)
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT4569950.8109171294490048No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA3559520.6316208581311221No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT3549240.629796718241028No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT3524470.6254013928443711No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATG3426610.6080365747855566No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC3403930.6040121105144151No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3263620.5791147303608051No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATTCGTATGCCG2952570.523920303044289No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA2914990.5172519006055986No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT2844430.5047313450953802No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT2585820.4588421605645195No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2578080.45746873228151086Illumina Single End Adapter 1 (95% over 22bp)
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC2451780.43505736378745524No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG2228790.3954887884866678No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCC2131230.37817720408223343No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATTCGTATGC2055020.3646540814145218No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1882270.3340003687672684No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT1771420.3143305334737921No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC1666190.29565794197237116No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATTCGTATGC1519040.26954683449889305No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATTCG1365130.24223619534671495No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT1355840.24058772651607538No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1320210.23426534283085607No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1274880.22622173765401093No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATG1235830.21929249030885758No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGT1221880.21681712538017925No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC1151600.20434625461404915No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT1147750.20366308938283686No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1137100.2017732946523405No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG1084870.19250531542475124No Hit
TTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATGC1054230.18706838485738891No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT1009090.17905849432831789No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA1004680.17827595960892925Illumina Single End Adapter 1 (95% over 22bp)
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG978320.17359849584405748No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATGCCGTCT861220.1528196260843274No Hit
TAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTA847190.150330065514481No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATG844010.14976578877805108No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATGCCG825100.14641029409695377No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG809810.14369715217871062No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTATG760740.13498990077725925No Hit
TTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTCGTAT753200.13365196159717074No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA714830.1268433772019458No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCC696410.12357483082300277No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTCTGTAGGCACCATCAATTCGTA656570.11650540152131493No Hit
AAGAGGTGCACAATCGACCGATCCTGCCTGTAGGCACCATCAATTCGTAT638130.11323330623207989No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA624700.11085021297099384No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT623020.11055210450646485No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC615870.10928336907707056No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATTCGTATGCC611940.10858600820469022No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTA606670.10765087034274505No Hit
TATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTAT594840.10555168990501998No Hit
TTTACCCAATCCTAATTCAGGGAGGTAGCTGTAGGCACCATCAATTCGTA586260.10402920738974684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)