FastQCFastQC Report
Sun 1 Oct 2023
SRR7471309.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471309.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11042064
Total Bases563.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT122436911.088225896897537No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT8622537.808802774553743No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA6939966.285020626578509No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG3553623.218257021513369No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC2322092.1029492312306832No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT1738321.5742708971800925No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG1559541.4123627611649416No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA1539271.3940056858935068No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT1529041.3847411136178889No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT1451531.3145459037368377No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC1375471.2456638541490068No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT1192901.0803233888157142No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1181311.069827162747834No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG1023130.9265749591742993No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG931160.8432843714725798No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA829210.7509556184423491No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT781240.7075126534314599No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG739600.6698023123213197No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG654590.5928148940270588No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG628190.5689063204125605No Hit
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGCC584130.5290043600544245No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT548500.4967368419527364No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC526150.4764960608813714No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC481160.4357518666799975No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG472600.4279996928110542No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA424410.38435748968671074Illumina Single End Adapter 1 (95% over 22bp)
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT413410.3743955840140032No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC386700.3502062657850924No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT363280.32899646298010954No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATTCGTATGC339890.3078138290087795No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATTCGTATGC330430.299246590130251No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC318120.2880983120547028No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC302500.2739524059994581No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATG302420.2738799557763838No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG294490.2666983274141501No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA287960.2607845779557155No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG286710.25965254322018055No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTAT266160.24104189216798597No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG234280.21217047827290259No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA231740.2098701836902956No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT216660.19621331664080194No Hit
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTATG213330.19319757610533683No Hit
AAGAGGTGCACAATCGACCGATCCTGATCTGTAGGCACCATCAATTCGTA211080.1911599135813739No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT200720.18177760969326023No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA199600.1807633065702209No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATG178540.16169078534592807No Hit
TGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGT173110.15677322645476424No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT168190.1523175377356987No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT165170.14958254181464625No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC160750.14557966698979466No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT157060.14223790045049547No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATTCGTATGC147560.13363443646042986No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATTCGTATGCC143540.12999381275094946No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATTCG136650.12375403728868081No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGT136510.12362724939830091No Hit
TGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTATGC131210.11882742211963271No Hit
GTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT128940.11677164703990123No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT127440.1154132053572593No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATG123530.11187220070450599No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTA120030.10870250344500812No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT119550.10826780210656267No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGTA119120.10787838215753866No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)